Regulation of RANTES/CCL5 expression in human astrocytes by interleukin-1 and interferon-beta

Journal of Neurochemistry
Mee-Ohk KimSunhee C Lee

Abstract

In the CNS, astrocytes are significant sources of RANTES/CCL5 (regulated upon activation, normal T cell expressed and secreted), a CC-chemokine with important biological function. Astrocyte RANTES/CCL5 has been shown to be induced by interleukin-1 (IL-1), with interferon-gamma (IFNgamma) as a primer, but whether type I interferons play any role in the expression of RANTES/CCL5 is not known. In this report, we studied the detailed mechanism of RANTES/CCL5 induction in primary human astrocytes activated with IL-1 and IFNbeta. Ribonuclease protection assay and ELISA showed that IFNbeta, although not effective alone, increased IL-1-induced RANTES/CCL5 expression, but did not antagonize IFNgamma. IL-1 or IL-1/IFNbeta-induced RANTES/CCL5 expression was inhibited by the super-repressor IkappaBalpha or inhibitors of p38 or c-Jun N-terminal kinase (JNK) MAPKs (mitogen-activated protein kinases), but not by extracellular signal regulated kinases (ERK) inhibitors. IFNbeta enhanced IL-1-induced phosphorylation of p38 MAPK, but was not effective alone. Transfection with mutated RANTES/CCL5 promoter-reporter constructs revealed that kappaB, interferon-stimulated response element (ISRE) and CAATT-enhancer binding protein-beta (C/EBPbeta) site...Continue Reading

Citations

Sep 26, 2008·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Nazira El-HageKurt F Hauser
Feb 3, 2007·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Juliana Croitoru-LamouryBruce J Brew
Apr 3, 2014·PloS One·Sung S ChoiSeung U Kim
Apr 17, 2014·BioMed Research International·Eric J GonzalezMargaret A Vizzard
Jan 10, 2012·Brain Research·A FouilletM N Woodroofe
Nov 28, 2006·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Lynette H ThomasJon S Friedland
Jun 27, 2006·European Journal of Clinical Investigation·N A C H Brabers, H S L M Nottet
Jul 6, 2015·Progress in Neurobiology·Marta Pulido-SalgadoJosep Saura
Mar 26, 2015·Brain : a Journal of Neurology·Candice ChapoulyGareth R John
Sep 24, 2013·JAK-STAT·Isabella RauchThomas Decker
Jan 31, 2017·The Journal of Investigative Dermatology·Ahmed F RezkVitali Alexeev
Jan 5, 2019·Reproductive Sciences·S Joseph HuangCharles J Lockwood
Oct 4, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sen Rong YanAndrew W Stadnyk
Nov 19, 2015·Frontiers in Microbiology·Kathleen Borgmann, Anuja Ghorpade
Jan 7, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Dilip KumarPeter J Nelson
Feb 23, 2018·Journal of Neuroinflammation·Arsalan AlizadehSoheila Karimi-Abdolrezaee
Oct 4, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Azeb Tadesse ArgawGareth R John
Oct 20, 2018·Pharmacological Reports : PR·Sanam DolatiMehdi Yousefi
Jan 18, 2019·Viruses·James A Carroll, Bruce Chesebro
Aug 21, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hongwei QinEtty N Benveniste
Nov 28, 2019·Seminars in Immunology·Ben X Wang, Eleanor N Fish
Dec 5, 2020·Brain, Behavior, and Immunity·Deanna NeculaMiou Zhou
Jul 19, 2005·Cytokine & Growth Factor Reviews·Mieke GouwyJo Van Damme

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