Regulation of rat cardiac myocyte growth by a neuronal factor secreted by PC12 cells

Pediatric Research
D L AtkinsS H Green

Abstract

Sympathetic innervation of cardiac myocytes in vitro induces growth independent of anatomic contact between the neurons and myocytes and is not mediated by alpha- or beta-adrenergic receptor stimulation. To establish a model system that will allow purification and identification of the neuronal factor(s) responsible for mediating this regulation, we have initiated studies utilizing conditioned medium from the PC12 cell line. PC12 cells acquire a cholinergic sympathetic neuronal phenotype when exposed to nerve growth factor. Culture medium conditioned by neuronal PC12 cells, but not nonneuronal PC12 cells, induces growth in newborn rat cardiac myocytes as measured by surface area and [35S]methionine incorporation into protein and increases expression of atrionatriuretic peptide, a marker for myocyte hypertrophy. The magnitude of the growth response is dose-dependent and mimics the response to sympathetic innervation. The myocyte response to conditioned medium is not detectable after 24 h of exposure; maximal rate of protein synthesis is obtained within 48 h. Neuronally differentiated PC12 cell-conditioned medium stimulation of growth could not be mimicked by alpha- or beta-adrenergic agonists or muscarinic agonists, nor inhibite...Continue Reading

References

Jul 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·L A Greene, A S Tischler
Dec 11, 1992·Journal of Molecular and Cellular Cardiology·D L AtkinsW J Marvin
Jan 20, 1992·Brain Research·J BaffiM Palkovits
Oct 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J SadoshimaS Izumo
Sep 1, 1992·The American Journal of Physiology·A M SamarelG L Engelmann
Aug 1, 1992·The American Journal of Physiology·S N AlloH E Morgan
Jan 1, 1992·Annual Review of Physiology·K M BakerD E Dostal
May 20, 1991·Brain Research. Developmental Brain Research·J E García-Arrarás
Sep 1, 1991·The American Journal of Physiology·W A ClarkR S Decker
Dec 1, 1991·Cell Regulation·C S LongP C Simpson
Jan 1, 1991·Circulation·H E Morgan, K M Baker
Dec 1, 1991·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·K R ChienS Chien
Jan 1, 1991·Annual Review of Physiology·T G Parker, M D Schneider
Mar 1, 1990·Journal of Molecular and Cellular Cardiology·T R Lloyd, W J Marvin
Feb 1, 1988·Journal of Molecular and Cellular Cardiology·G L Engelmann, R G Gerrity
Sep 15, 1987·Biochemical and Biophysical Research Communications·S L MulvaghM D Schneider
Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·S IzumoV Mahdavi
Sep 1, 1987·Molecular and Cellular Biology·D G LeonardL A Greene
Jul 1, 1984·Circulation Research·W J MarvinK Hermsmeyer
Apr 1, 1984·Circulation Research·E W ThompsonG Cooper
Mar 27, 1995·Annals of the New York Academy of Sciences·G LemboK R Chien
Dec 1, 1993·Circulation Research·W A ClarkM C LaPointe
Jan 1, 1993·Annual Review of Physiology·K R ChienS M Evans
Jul 1, 1993·Circulation Research·P SilS Sen

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Citations

Dec 17, 2009·Mediators of Inflammation·Hanssa Summah, Jie-Ming Qu
May 31, 2012·International Journal of Inflammation·Lea KyhäläHeikki Repo
Oct 6, 2001·Journal of Cardiovascular Pharmacology·D H Damon
Feb 13, 2001·American Journal of Physiology. Heart and Circulatory Physiology·W ZhengR J Tomanek

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