Regulation of rat dopamine beta-hydroxylase gene transcription by early growth response gene 1 (Egr1)

Brain Research
Shu-Yuan ChengEsther L Sabban

Abstract

Egr1, a transcription factor rapidly induced by various stimuli including stress, can elevate transcription of genes for the catecholamine biosynthetic enzymes TH and PNMT. To examine if Egr1 also regulates dopamine beta-hydroxylase (DBH) gene expression, PC12 cells were transfected with expression vector for full length or truncated inactive Egr1 and various DBH promoter-driven luciferase constructs. While Egr1 elevated TH promoter activity, DBH promoter activity was reduced. The reduction occurred as early as 4 h and reached maximal inhibition 16-40 h after transfection. Egr1 also reduced the expression of endogenous DBH mRNA and the induction of DBH promoter activity by cAMP. These effects were not observed with truncated Egr1 lacking the DNA binding domain. The first 247, but not 200, nucleotides of DBH promoter are sufficient for this suppression. Several putative Egr1 motifs were identified, and mutagenesis showed that the motif at -227/-224 is required. Binding of Egr1 to this region of the DBH promoter was verified by chromatin immunoprecipitation and electrophoretic mobility shift assays. This study demonstrates that DBH promoter contains at least one functional Egr1 motif; and indicates, for the first time, that Egr1 ...Continue Reading

References

Jun 1, 1992·The American Journal of Psychiatry·A F Schatzberg, A J Rothschild
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·B Christy, D Nathans
Jun 21, 1994·Proceedings of the National Academy of Sciences of the United States of America·B NankovaE L Sabban
Oct 1, 1996·Proceedings of the National Academy of Sciences of the United States of America·D M Cohen
Jul 21, 1998·Annals of the New York Academy of Sciences·R Kvetnanský, E L Sabban
Sep 3, 1998·The Journal of Biological Chemistry·D J SwansonE J Lewis
Mar 18, 1999·The American Journal of Pathology·E S Silverman, T Collins
Jun 8, 2000·The Journal of Biological Chemistry·N A Papanikolaou, E L Sabban
Oct 18, 2002·Journal of Cellular Physiology·Gerald Thiel, Giuseppe Cibelli
Feb 6, 2003·American Journal of Physiology. Renal Physiology·Xianghong Zhang, Youhua Liu
Apr 3, 2003·Brain Research. Molecular Brain Research·Akira NakashimaEsther L Sabban
Dec 6, 2005·Pharmacology, Biochemistry, and Behavior·Shu-Yuan ChengEsther L Sabban
May 13, 2006·Cellular and Molecular Neurobiology·Esther L SabbanRichard Kvetnansky

❮ Previous
Next ❯

Citations

Nov 11, 2010·Journal of Neuroendocrinology·L I SerovaE L Sabban
Apr 4, 2009·Physiological Reviews·Richard KvetnanskyMiklos Palkovits
Oct 16, 2019·Journal of Neurochemistry·Eugene Gonzalez-Lopez, Kent E Vrana
Jul 18, 2018·Frontiers in Endocrinology·Collin J ByrneT C Tai
Feb 7, 2021·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Yunjeong SongJaesuk Yun

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.