Regulation of RAW264.7 macrophage polarization on smooth and rough surface topographies by galectin-3

Journal of Biomedical Materials Research. Part a
F KianoushDonald Maxwell Brunette

Abstract

Recognition of topographical features induces phenotypic changes in macrophages although the receptors and signaling pathways are not completely characterized. As integrin molecules in focal adhesions/podosomes are in intimate contact with topography and topography modulates the NFkB pathway through cholesterol enriched raft-associated adhesive signaling structures we hypothesized that a cell-surface signaling complex comprised of galectin-3 together with its ligand CD98 and integrinβ1 is important for topography-directed lineage determination. This study used polished, sand blasted and acid etched (SLA) surfaces and two novel grooved topographies (G1 and G2) produced by anisotropic etching of Si <1 1 0> to evaluate the role of galectin-3 in macrophage polarization in RAW 264.7 macrophages, as determined by gene expression and morphology. In the presence of the galectin-3 inhibitor, lactose, the M2 marker (mannose receptor) was down-regulated while the M1 marker (iNOS) was up-regulated on smooth and rough surfaces. This skewing of phenotype suggests a role for galectin-3 in macrophage polarization towards the M2 phenotype. Additionally, we evaluated the role of PI3K on polarization using PI3K inhibitor LY294002. We found that t...Continue Reading

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Citations

Dec 14, 2017·Scientific Reports·Svenja F B MennensKoen van den Dries
Aug 3, 2019·Clinical Implant Dentistry and Related Research·Yulan WangYufeng Zhang
Feb 12, 2020·Journal of Cellular and Molecular Medicine·Lingbo KongLiang Yan
Feb 19, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Mingzheng LiHuipin Yuan

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