Regulation of renal citrate metabolism by bicarbonate ion and pH: observations in tissue slices and mitochondria

The Journal of Clinical Investigation
D P Simpson

Abstract

The effect of acid-base balance on the oxidation and utilization of citrate and other organic acids has been studied in tissue slices and isolated kidney mitochondria. The results show that: 1) With bicarbonate-buffered media, citrate oxidation and utilization are inhibited in slices of renal cortex and in kidney mitochondria when [HCO(3) (-)] and pH are increased within the physiologic range (pH 7.0 to 7.8; 10 to 60 mumoles HCO(3) (-) per ml). When phosphate or Tris buffers are used, no comparable effect on citrate oxidation occurs when pH is varied. 2) This effect is not demonstrable in heart or liver slices when a physiologic buffer is used. 3) alpha-Ketoglutarate utilization is inhibited in slices of renal cortex under similar conditions. Pyruvate and L-malate utilization are not inhibited in slices or mitochondria. 4) Citrate content in slices of renal cortex incubated with a high [HCO(3) (-)] is considerably greater than the concentration found with a low [HCO(3) (-)] in the medium. This effect is not duplicated by pH change in a nonbicarbonate buffer system. In mitochondria citrate content is also increased markedly at high bicarbonate concentrations. 5) The kinetic characteristics of the inhibition of citrate oxidation ...Continue Reading

References

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Citations

Jun 29, 1994·Molecular and Cellular Biochemistry·M M SolaA Vargas
Jan 1, 1984·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·M J Haas, D P Simpson
Jan 13, 2000·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·T OzekiS Furuyama
Sep 18, 1980·The New England Journal of Medicine·D RudmanM H Kutner
Jun 16, 2006·Current Opinion in Nephrology and Hypertension·Orson W Moe, Patricia A Preisig
Aug 6, 2010·American Journal of Physiology. Renal Physiology·Michael F Romero
Sep 1, 1970·The Journal of Clinical Investigation·S Adler
Aug 1, 1975·The Journal of Clinical Investigation·S AdlerD S Fraley
Jul 6, 2013·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Anneli StrobelFelix C Mark
Jun 1, 1969·The Journal of Clinical Investigation·D P Simpson, D J Sherrard
Feb 1, 1980·Archives internationales de physiologie et de biochimie·H SimonnetM Pellet
May 1, 1972·Kidney International·A S Relman
May 19, 2009·Journal of Renal Nutrition : the Official Journal of the Council on Renal Nutrition of the National Kidney Foundation·Abbas BasiriHamid Pakmanesh
Feb 1, 1975·Metabolism: Clinical and Experimental·S Adler
Jun 13, 2018·Current Opinion in Urology·Dimitrios KotsirisPanagiotis Kallidonis
Aug 28, 2019·Annual Review of Marine Science·Frank MelznerLars Tomanek
Aug 1, 1993·The Journal of Urology·K SakhaeeC Y Pak
Mar 1, 1989·The Journal of Urology·V M Buckalew
May 9, 1972·Biochimica Et Biophysica Acta·G RoriveA Kleinzeller
Jun 20, 1973·Biochimica Et Biophysica Acta·K Kurokawa, H Rasmussen
Apr 22, 1969·Biochimica Et Biophysica Acta·J S Britten
Feb 27, 1973·Biochimica Et Biophysica Acta·D P Simpson, S Angielski
Jun 20, 1973·Biochimica Et Biophysica Acta·K Kurokawa, H Rasmussen
Oct 1, 1972·Archives of Biochemistry and Biophysics·G Rorive, A Kleinzeller
Oct 1, 1968·Biochemistry·N Nagata, H Rasmussen
Jan 1, 1984·Kidney International·R L Tannen, S Sastrasinh
Sep 1, 1978·Kidney International·A GougouxP Vinay

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