Regulation of renal vitamin D-24-hydroxylase by phosphate: effects of hypophysectomy, growth hormone and insulin-like growth factor I

Biochemical and Biophysical Research Communications
S WuA J Brown

Abstract

Dietary phosphate (Pi) regulates the production of 1,25-dihydroxyvitamin D3. This control is blunted in hypophysectomized (HPX) rats but can be restored by growth hormone (GH) or IGF-I. The regulation of the vitamin D catabolism by Pi, GH and IGF-I is less clear. In the present study, we found that the activity and transcript levels of the catabolic enzyme, vitamin D-24-hydroxylase (24-OHase) were decreased 3- and 5-fold, respectively, during Pi restriction in normal rats, but this effect is greatly reduced in HPX rats. Examination of the serum chemistries revealed that HPX rats on the high Pi diet had lower serum Pi levels than normal rats on this diet, presumably due to the known defective reabsorption of Pi by HPX rats. Treatment of HPX rats, adapted to a 0.6% P diet, with GH (150 micrograms) or IGF-I (80 micrograms) suppressed 24-OHase mRNA levels by 88% and 64%, respectively, by 20 hours and these effects were preceded by decreases in serum Pi. Our findings show that the 24-OHase is regulated by dietary Pi and this control is modulated by hypophysectomy, GH and IGF-I.

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Citations

Mar 8, 2013·Seminars in Nephrology·Cortney Bosworth, Ian H de Boer
Sep 15, 2011·Molecular and Cellular Endocrinology·Farzana Perwad, Anthony A Portale
Jul 1, 2004·The Journal of Steroid Biochemistry and Molecular Biology·M A Tryfonidou, H A W Hazewinkel
Aug 5, 2017·Clinical Journal of the American Society of Nephrology : CJASN·Zona BatacchiIan H de Boer
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Apr 3, 2012·International Urology and Nephrology·Hulya Taskapan
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Mar 12, 2020·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Charles GinsbergIan H de Boer
Jan 11, 2013·Physiological Reviews·Sascha Kopic, John P Geibel
Dec 9, 2020·Metabolites·Rafael Vessecchi Amorim ZafalonMarcio Antonio Brunetto
May 6, 2003·The Journal of Nutrition·Peter D Gluckman, Catherine S Pinal

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