Complex regulatory networks regulate stem cell behavior and contributions to tissue growth, repair, and homeostasis. A full understanding of the networks controlling stem cell self-renewal and differentiation, however, has not yet been realized. To systematically dissect these networks and identify their components, we performed an unbiased, transcriptome-wide in vivo RNAi screen in female Drosophila germline stem cells (GSCs). Based on characterized cellular defects, we classified 646 identified genes into phenotypic and functional groups and unveiled a comprehensive set of networks regulating GSC maintenance, survival, and differentiation. This analysis revealed an unexpected role for ribosomal assembly factors in controlling stem cell cytokinesis. Moreover, our data show that the transition from self-renewal to differentiation relies on enhanced ribosome biogenesis accompanied by increased protein synthesis. Collectively, these results detail the extensive genetic networks that control stem cell homeostasis and highlight the intricate regulation of protein synthesis during differentiation.
The Rho GTPase and a putative RhoGEF mediate a signaling pathway for the cell shape changes in Drosophila gastrulation
A discrete transcriptional silencer in the bam gene determines asymmetric division of the Drosophila germline stem cell
Dpp signaling silences bam transcription directly to establish asymmetric divisions of germline stem cells
Pelota controls self-renewal of germline stem cells by repressing a Bam-independent differentiation pathway
Diet controls normal and tumorous germline stem cells via insulin-dependent and -independent mechanisms in Drosophila
A hierarchical network controls protein translation during murine embryonic stem cell self-renewal and differentiation
Specific roles of Target of rapamycin in the control of stem cells and their progeny in the Drosophila ovary
Ribosome profiling of mouse embryonic stem cells reveals the complexity and dynamics of mammalian proteomes
rRNA pseudouridylation defects affect ribosomal ligand binding and translational fidelity from yeast to human cells
Drosophila Piwi functions downstream of piRNA production mediating a chromatin-based transposon silencing mechanism in female germ line
Drosophila Tel2 is expressed as a translational fusion with EpsinR and is a regulator of wingless signaling
A transcriptome-wide RNAi screen in the Drosophila ovary reveals factors of the germline piRNA pathway
Aurora B and cyclin B have opposite effects on the timing of cytokinesis abscission in Drosophila germ cells and in vertebrate somatic cells
Drosophila Spag is the homolog of RNA polymerase II-associated protein 3 (RPAP3) and recruits the heat shock proteins 70 and 90 (Hsp70 and Hsp90) during the assembly of cellular machineries.
Correction: ALIX and ESCRT-III Coordinately Control Cytokinetic Abscission during Germline Stem Cell Division In Vivo
Whole-animal genome-wide RNAi screen identifies networks regulating male germline stem cells in Drosophila
The ubiquitin hybrid gene UBA52 regulates ubiquitination of ribosome and sustains embryonic development
Female Mimicry by Sneaker Males Has a Transcriptomic Signature in Both the Brain and the Gonad in a Sex-Changing Fish
Aldehyde dehydrogenase activity helps identify a subpopulation of murine adipose-derived stem cells with enhanced adipogenic and osteogenic differentiation potential
Spaghetti, a homolog of human RPAP3 (RNA polymerase II-associated protein 3), determines the fate of germline stem cells in Drosophila ovary
Protein synthesis and degradation are essential to regulate germline stem cell homeostasis in Drosophila testes
DNA damage-induced Lok/CHK2 activation compromises germline stem cell self-renewal and lineage differentiation
The replicative histone chaperone CAF1 is essential for the maintenance of identity and genome integrity in adult stem cells
Whole genome screen reveals a novel relationship between Wolbachia levels and Drosophila host translation
Tip60 complex promotes expression of a differentiation factor to regulate germline differentiation in female Drosophila
Regulation of mitochondrion-associated cytosolic ribosomes by mammalian mitochondrial ribonuclease T2 (RNASET2)
Ribosome Incorporation into Somatic Cells Promotes Lineage Transdifferentiation towards Multipotency
scRNA-Seq reveals distinct stem cell populations that drive hair cell regeneration after loss of Fgf and Notch signaling
Sex-dependent and -independent transcriptional changes during haploid phase gametogenesis in the sugar kelp Saccharina latissima
Molecular profiling of stem cell-like female germ line cells in Drosophila delineates networks important for stemness and differentiation
Cell Fate Control by Translation: mRNA Translation Initiation as a Therapeutic Target for Cancer Development and Stem Cell Fate Control
Identification of Breast Cancer Stem Cell Related Genes Using Functional Cellular Assays Combined With Single-Cell RNA Sequencing in MDA-MB-231 Cells
Heads or tails? Differential translational regulation in cercarial heads and tails of schistosome worms
RpS13 controls the homeostasis of germline stem cell niche through Rho1-mediated signals in the Drosophila testis
Functional Interactions Between rsks-1 /S6K, glp-1 /Notch, and Regulators of Caenorhabditis elegans Fertility and Germline Stem Cell Maintenance
Efficient Expression of Genes in the Drosophila Germline Using a UAS Promoter Free of Interference by Hsp70 piRNAs
Srlp is crucial for the self-renewal and differentiation of germline stem cells via RpL6 signals in Drosophila testes
Co-expression network analysis identified key genes in association with mesenchymal stem cell osteogenic differentiation
The Flemmingsome reveals an ESCRT-to-membrane coupling via ALIX/syntenin/syndecan-4 required for completion of cytokinesis.
microRNAs Mediated Regulation of the Ribosomal Proteins and its Consequences on the Global Translation of Proteins.
An efficient Chlorella sp.-Cupriavidus necator microcosm for phenol degradation and its cooperation mechanism
Decoding mixed messages in the developing cortex: translational regulation of neural progenitor fate.
Identification of co-expression network correlated with different periods of adipogenic and osteogenic differentiation of BMSCs by weighted gene co-expression network analysis (WGCNA).
Single-Cell Atlas Reveals Fatty Acid Metabolites Regulate the Functional Heterogeneity of Mesenchymal Stem Cells.
Adult Stem Cells
Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.