Regulation of Ribosome Biogenesis and Protein Synthesis Controls Germline Stem Cell Differentiation

Cell Stem Cell
Carlos G SanchezRuth Lehmann

Abstract

Complex regulatory networks regulate stem cell behavior and contributions to tissue growth, repair, and homeostasis. A full understanding of the networks controlling stem cell self-renewal and differentiation, however, has not yet been realized. To systematically dissect these networks and identify their components, we performed an unbiased, transcriptome-wide in vivo RNAi screen in female Drosophila germline stem cells (GSCs). Based on characterized cellular defects, we classified 646 identified genes into phenotypic and functional groups and unveiled a comprehensive set of networks regulating GSC maintenance, survival, and differentiation. This analysis revealed an unexpected role for ribosomal assembly factors in controlling stem cell cytokinesis. Moreover, our data show that the transition from self-renewal to differentiation relies on enhanced ribosome biogenesis accompanied by increased protein synthesis. Collectively, these results detail the extensive genetic networks that control stem cell homeostasis and highlight the intricate regulation of protein synthesis during differentiation.

References

Dec 1, 1990·Genes & Development·Dennis McKearin, Allan C Spradling
Mar 4, 1988·Science·Lynn CooleyAllan C Spradling
Mar 24, 1999·The Journal of Cell Biology·E GiordanoMaria Furia
Apr 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Toshie Kai, Allan C Spradling
Feb 22, 2005·Nature Cell Biology·Azadeh ArabiAnthony P H Wright
May 23, 2006·Current Biology : CB·David A GuertinDavid M Sabatini
Dec 28, 2007·Cell·Hiroyuki TakaiTitia de Lange
Oct 9, 2009·Journal of Cell Science·Mathieu Laplante, David M Sabatini
Apr 30, 2010·The Journal of Biological Chemistry·Takeshi KaizukaNoboru Mizushima
Apr 5, 2011·Nature Methods·Jianquan NiNorbert Perrimon
Jul 28, 2011·Cold Spring Harbor Perspectives in Biology·Allan C SpradlingShosei Yoshida
Dec 14, 2011·Proceedings of the National Academy of Sciences of the United States of America·Jing LiuAdrian Salic
Dec 14, 2011·Proceedings of the National Academy of Sciences of the United States of America·Sidney H Wang, Sarah C R Elgin
May 24, 2012·Nature Reviews. Molecular Cell Biology·Shifeng Xue, Maria Barna
Feb 28, 2013·Science Signaling·Arunachalam VinayagamNorbert Perrimon
Nov 6, 2013·Genetics·John L Woolford, Susan J Baserga
Mar 1, 2014·Developmental Cell·Dong YanNorbert Perrimon
Mar 29, 2014·Nature·Robert A J SignerSean J Morrison
Oct 11, 2014·Cell·Michael BuszczakSean J Morrison

Citations

Jun 1, 2016·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Matthew L KrausharMladen-Roko Rasin
Mar 23, 2017·ACS Applied Materials & Interfaces·Anqi SunXiangang Hu
Jun 27, 2017·Nature Cell Biology·Fengbiao ZhouCarsten Müller-Tidow
May 31, 2018·Development, Growth & Differentiation·Shumpei MoritaSatoru Kobayashi
Oct 5, 2016·Molecular and Cellular Biology·Anne-Marie Landry-VoyerFrançois Bachand
Sep 12, 2018·Journal of Biochemistry·Kayoko Tsuda-Sakurai, Masayuki Miura
May 30, 2019·Molecular and Cellular Biology·Gangarao DavuluriSrinivasan Dasarathy
Dec 17, 2019·PLoS Genetics·Zhejun JiNicholas E Baker
Feb 27, 2020·Cells·Mathieu GabutSébastien Durand
Apr 25, 2020·The Journal of Cell Biology·Dan Li, Jianlong Wang
May 27, 2020·Current Opinion in Hematology·Bernadette A Chua, Robert A J Signer
Feb 5, 2020·Experimental and Therapeutic Medicine·Qiang ZhouHao Cheng
Aug 6, 2016·Genes & Development·Robert A J SignerSean J Morrison
May 11, 2018·ELife·Kevin F ChauMaria K Lehtinen
Apr 21, 2018·Oncogenesis·Amandine Bastide, Alexandre David
Aug 8, 2018·Cold Spring Harbor Perspectives in Biology·Felipe Karam Teixeira, Ruth Lehmann
Jan 31, 2019·Frontiers in Genetics·Soroush TahmasebiNahum Sonenberg
Jul 23, 2020·Stem Cells International·Zhenzhen HanWei Li
Nov 18, 2018·Frontiers in Immunology·Wendi A BaconD Stephen Charnock-Jones
Dec 20, 2018·Epigenomics·Katerina GrafanakiConstantinos Stathopoulos
Jan 6, 2019·Nature Protocols·Lorena Hidalgo San Jose, Robert A J Signer
Oct 24, 2019·Cell Communication and Signaling : CCS·Filip VujovicRamin M Farahani
Mar 14, 2019·Nature Communications·Samuel C KimmeySean C Bendall
May 27, 2016·G3 : Genes - Genomes - Genetics·Elizabeth T AblesDaniela Drummond-Barbosa
Mar 25, 2017·Scientific Reports·Rosario VicidominiMaria Furia
Jun 13, 2020·Mechanisms of Ageing and Development·Samim SharifiHolger Bierhoff
Feb 8, 2020·Cell Stem Cell·Bernadette A ChuaRobert A J Signer
Feb 11, 2021·Wiley Interdisciplinary Reviews. RNA·Karl NorrisJulie Louise Aspden
Jul 15, 2020·The Science of the Total Environment·Tao YiNigel W T Quinn
Feb 21, 2021·Current Opinion in Insect Science·Ruei-Jiun HungNorbert Perrimon
Nov 2, 2020·Current Opinion in Neurobiology·Mariah L Hoye, Debra L Silver
Apr 30, 2021·Frontiers in Cell and Developmental Biology·Jiayi XieMeng Zhao
May 8, 2021·Stem Cell Reports·Shenae L CafeTessa Lord
May 14, 2021·Trends in Molecular Medicine·Pia SommerkampAndreas Trumpp

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.