Regulation of ROCK1 via Notch1 during breast cancer cell migration into dense matrices.

BMC Cell Biology
Vanisri RavirajLilian Soon

Abstract

The behaviour of tumour cells depends on factors such as genetics and the tumour microenvironment. The latter plays a crucial role in normal mammary gland development and also in breast cancer initiation and progression. Breast cancer tissues tend to be highly desmoplastic and dense matrix as a pre-existing condition poses one of the highest risk factors for cancer development. However, matrix influence on tumour cell gene expression and behaviour such as cell migration is not fully elucidated. We generated high-density (HD) matrices that mimicked tumour collagen content of 20 mg/cm3 that were ~14-fold stiffer than low-density (LD) matrix of 1 mg/cm3. Live-cell imaging showed breast cancer cells utilizing cytoplasmic streaming and cell body contractility for migration within HD matrix. Cell migration was blocked in the presence of both the ROCK inhibitor, Y-27632, and the MMP inhibitor, GM6001, but not by the drugs individually. This suggests roles for ROCK1 and MMP in cell migration are complicated by compensatory mechanisms. ROCK1 expression and protein activity, were significantly upregulated in HD matrix but these were blocked by treatment with a histone deacetylase (HDAC) inhibitor, MS-275. In HD matrix, the inhibition of ...Continue Reading

References

Jan 1, 1989·Advances in Cancer Research·B J Rollins, C D Stiles
Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·P K ElderM J Getz
Apr 14, 1999·Proceedings of the National Academy of Sciences of the United States of America·A SaitoO Nakanishi
Jul 30, 1999·Journal of Medicinal Chemistry·T SuzukiO Nakanishi
Nov 5, 1999·The Journal of Biological Chemistry·M AmanoK Kaibuchi
Jun 27, 2000·Biophysical Journal·C M LoY L Wang
Jan 1, 1996·Journal of Mammary Gland Biology and Neoplasia·D Medina
Jul 19, 2000·Current Biology : CB·A G Petcherski, J Kimble
Dec 9, 2000·Developmental Biology·J S Mumm, R Kopan
Mar 21, 2001·The Journal of Experimental Medicine·E Y LinJ W Pollard
Jan 5, 2002·Biomaterials·Laurence BesseaMarie-Madeleine Giraud-Guille
Mar 15, 2002·Current Opinion in Genetics & Development·Tony Kouzarides
May 11, 2002·Science·Bryony S Wiseman, Zena Werb
Jan 28, 2003·Journal of Cellular Physiology·Tatsuya IsoYasuo Hamamori
May 2, 2003·Nature Reviews. Cancer·Peter Friedl, Katarina Wolf
Jun 5, 2003·Nature Reviews. Molecular Cell Biology·Kirsi Riento, Anne J Ridley
Aug 21, 2003·Breast Cancer Research : BCR·Salem AlowamiPeter H Watson
Sep 17, 2003·The Journal of Pharmacology and Experimental Therapeutics·Erding HuMichael Jaye
Apr 8, 2004·Developmental Cell·Rowena McBeathChristopher S Chen
Dec 14, 2004·Biochimica Et Biophysica Acta·Salvador A BenitahJuan Carlos Lacal
Feb 22, 2005·Nature Cell Biology·Simon WilkinsonChristopher J Marshall
Mar 2, 2005·Journal of Virological Methods·Shongshan FanStephen Dewhurst
Apr 20, 2005·Journal of Mammary Gland Biology and Neoplasia·Matthew J Paszek, Valerie M Weaver
Apr 27, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Qin C RyanEdward A Sausville
May 3, 2005·The Journal of Clinical Investigation·Kathryn E Wellen, Gökhan S Hotamisligil
May 14, 2005·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Teresa Gómez del PulgarJuan Carlos Lacal
Sep 20, 2005·Breast Cancer Research : BCR·Giske UrsinAnna H Wu
Sep 20, 2005·Cancer Cell·Matthew J PaszekValerie M Weaver
Dec 3, 2005·Cancer Research·Adriana AlbiniDouglas M Noonan
Mar 31, 2006·Nature Reviews. Cancer·Raghu Kalluri, Michael Zeisberg
Jul 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Muhammad H ZamanPaul Matsudaira
Dec 28, 2006·BMC Medicine·Paolo P ProvenzanoPatricia J Keely
Mar 14, 2007·Molecular and Cellular Biology·Takashi YugawaTohru Kiyono

❮ Previous
Next ❯

Citations

Jan 25, 2012·Clinical & Experimental Metastasis·Vanisri RavirajLilian Soon
Mar 13, 2014·Breast Cancer Research and Treatment·C W HuoE W Thompson
Aug 22, 2015·Journal of Pineal Research·Thaiz Ferraz BorinDebora Aparecida Pires de Campos Zuccari
Oct 1, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Peng ZhangYu Hua Zhou
Sep 15, 2014·Acta histochemica·Jenifer BottinoDebora Aparecida Pires de Campos Zuccari
Dec 4, 2012·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Marie Morgan-FisherAtsuko Yoneda
Feb 23, 2017·American Journal of Respiratory Cell and Molecular Biology·Su S HtweAmir M Ghaemmaghami
Jun 10, 2019·Breast Cancer Research and Treatment·Michael S ShawkyErik W Thompson
Jan 20, 2016·Oncotarget·Alastair J Ironside, J Louise Jones
Nov 8, 2013·American Journal of Physiology. Cell Physiology·Francois BordeleauCynthia A Reinhart-King

❮ Previous
Next ❯

Datasets Mentioned

BETA
GM6001

Methods Mentioned

BETA
confocal microscopy
PCR
xenograft
transfection
histone
scanning electron microscopy
protein assay

Software Mentioned

Basic Local Alignment Search Tool ( BLAST
SMART
Excel
ImageJ

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Related Papers

The Journal of Surgical Research
Buckminster FarrowDavid H Berger
Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
Flora AngeliJohn G Delinassios
© 2021 Meta ULC. All rights reserved