Abstract
To investigate the effect of JAKs-STATs signal pathway on expression of S100A4 in pulmonary arterial smooth muscle cells (PASMCs), the action of S100A4 and hypoxia induced factor 1 (HIF-1) on the proliferation of hypoxic PASMCs. The results showed that S100A4 immunostaining was localized in the cytoplasm and nuclei of PASMCs exposure to hypoxia and it was predominantly expressed in rhomboid cells (R-SMCs). The mRNA and protein levels of S100A4 expression increased in PASMCs after hypoxic stimulus for 4, 8, 16 h. The immunofluorescence intensity and protein levels of S100A4 were suppressed, and the number of R-SMCs was reduced, when pretreatment with HIF-1α siRNA, STAT3 siRNA, S100A4 siRNA, and S100A4 inhibitor NSC 95397. Pretreatment with HIF-1α siRNA and anti-IL-6 antibodies, the levels of phospho-JAK2, -STAT3, and S100A4 were decreased, while HIF-1α kept stable in hypoxic cells. Importantly, pretreatment with HIF-1α siRNA, anti-IL-6 antibodies, STAT3 siRNA, and S100A4 siRNA, significantly attenuated the proliferation of PASMCs exposure to hypoxia. These data demonstrate that S100A4 is predominantly expressed in hypoxic R-SMCs, and regulated by the activation of JAK2-STAT3 signal pathway, which is dependent on hypoxia-induced ...Continue Reading
References
Mar 8, 2000·Cytokine·G HartmannM Toepfer
Aug 29, 2001·Circulation·S NegoroK Yamauchi-Takihara
Jul 23, 2004·Physiological Reviews·Gary K OwensBrian R Wamhoff
Sep 15, 2004·Cardiovascular Research·Buddhadeb DawnRoberto Bolli
Jun 9, 2005·Cell Biology International·Guan-Song WangJi-Qing Zhao
Jun 18, 2005·Circulation Research·Jean-Marc HyvelinPaul McLoughlin
Jul 9, 2005·Circulation Research·Allan LawrieMarlene Rabinovitch
Aug 9, 2005·British Journal of Pharmacology·Lydia BellikAstrid Parenti
Sep 9, 2005·Frontiers in Bioscience : a Journal and Virtual Library·Thomas M LincolnChung-Sik Choi
Apr 28, 2006·Current Atherosclerosis Reports·Neeta AdhikariJennifer L Hall
Feb 27, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Weilin ZhouJ Usha Raj
Mar 10, 2007·Circulation Research·Anne C BrissetMarie-Luce Bochaton-Piallat
Oct 9, 2007·The International Journal of Biochemistry & Cell Biology·Guansong WangGuangyu Wu
Jan 26, 2008·Proteomics·Michaël G MagagninBradly G Wouters
Mar 7, 2008·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Mikael SchneiderSøren P Sheikh
Jun 13, 2008·International Journal of Cancer. Journal International Du Cancer·Kjetil BoyeGunhild M Maelandsmo
Aug 30, 2008·Clinical Science·Anthony J Muslin
Sep 11, 2008·Current Molecular Medicine·Mariam GrigorianEugene Lukanidin
Feb 28, 2009·Arthritis and Rheumatism·Raghunatha R YammaniRichard F Loeser
Mar 3, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Weilin ZhouJ Usha Raj
Jun 23, 2009·Journal of Molecular and Cellular Cardiology·Carolin KrausPatrick Most
Jul 21, 2009·Pharmacology & Therapeutics·David G S Farmer, Simon Kennedy
Aug 29, 2009·Circulation Research·Edda SpiekerkoetterMarlene Rabinovitch
Sep 11, 2009·American Journal of Physiology. Cell Physiology·Haifa A MadiKaren E Porter
Sep 22, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Maria G FridKurt R Stenmark
Oct 15, 2009·Respiratory Research·Seiichiro SakaoNorbert F Voelkel
Dec 19, 2009·The American Journal of Pathology·Kjetil Boye, Gunhild M Maelandsmo
Dec 29, 2009·Journal of Molecular and Cellular Cardiology·Magda Descorbeth, Madhu B Anand-Srivastava
Apr 7, 2010·Cancer Science·Ruixiu ZhangXiuju Sun
Apr 27, 2010·The International Journal of Biochemistry & Cell Biology·Yuncheng LiGuisheng Qian
Jun 17, 2010·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·Won Sun ParkEun A Ko
Sep 16, 2010·Biochimica Et Biophysica Acta·Guansong WangGuangyu Wu
Nov 26, 2010·The International Journal of Biochemistry & Cell Biology·Hongjin YinGuansong Wang
Dec 21, 2010·Cancer Research·Jeng-Fan LoYau-Hua Yu
Feb 4, 2011·American Journal of Physiology. Cell Physiology·Min ChenKathleen L O'Connor
Mar 9, 2011·Circulation·Roxane PaulinSébastien Bonnet
Jul 20, 2011·Cellular Signalling·Cheng SunQingzhou Meng
Citations
Jul 14, 2012·Journal of Neuroinflammation·Guansong WangMark A Decoster
Apr 23, 2013·Oncogene·M R PawlusC-J Hu
Mar 4, 2014·Biomaterials·Xueping LiuJianxiang Zhang
Mar 14, 2013·Biomaterials·Huaping ChenGuansong Wang
Dec 9, 2014·Journal of Proteomics·Chang-Peng XuBin Yu
Feb 26, 2013·PloS One·Ying LuoZhi-Chao Li
Jun 25, 2014·Scientific Reports·Yuki HasegawaHarukazu Suzuki
Nov 16, 2012·Circulation·Chris JoplingJuan Carlos Izpisua Belmonte
Apr 30, 2019·Journal of Cellular Physiology·Jie ZhuFei-Wu Kang
Oct 9, 2015·Journal of Cardiovascular Pharmacology·Ming-Xing LiYong Wang
Oct 15, 2020·American Journal of Respiratory Cell and Molecular Biology·Dinesh YeraboluRalph Theo Schermuly
Mar 12, 2021·Journal of Bioenergetics and Biomembranes·Zhi HuXiaohui Luo