Regulation of SCFTIR1/AFBs E3 ligase assembly by S-nitrosylation of Arabidopsis SKP1-like1 impacts on auxin signaling

Redox Biology
María José IglesiasClaudia Anahí Casalongué

Abstract

The F-box proteins (FBPs) TIR1/AFBs are the substrate recognition subunits of SKP1-cullin-F-box (SCF) ubiquitin ligase complexes and together with Aux/IAAs form the auxin co-receptor. Although tremendous knowledge on auxin perception and signaling has been gained in the last years, SCFTIR1/AFBs complex assembly and stabilization are emerging as new layers of regulation. Here, we investigated how nitric oxide (NO), through S-nitrosylation of ASK1 is involved in SCFTIR1/AFBs assembly. We demonstrate that ASK1 is S-nitrosylated and S-glutathionylated in cysteine (Cys) 37 and Cys118 residues in vitro. Both, in vitro and in vivo protein-protein interaction assays show that NO enhances ASK1 binding to CUL1 and TIR1/AFB2, required for SCFTIR1/AFB2 assembly. In addition, we demonstrate that Cys37 and Cys118 are essential residues for proper activation of auxin signaling pathway in planta. Phylogenetic analysis revealed that Cys37 residue is only conserved in SKP proteins in Angiosperms, suggesting that S-nitrosylation on Cys37 could represent an evolutionary adaption for SKP1 function in flowering plants. Collectively, these findings indicate that multiple events of redox modifications might be part of a fine-tuning regulation of SCFTI...Continue Reading

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Citations

Jan 23, 2019·Plant & Cell Physiology·Jing ZhangWeibiao Liao
Jun 8, 2019·Journal of Experimental Botany·Clara WilliamsAlain Goossens
Jul 16, 2019·The Plant Journal : for Cell and Molecular Biology·Md Sanaullah BiswasJun'ichi Mano
Jan 22, 2019·Journal of Integrative Plant Biology·Jian FengJianru Zuo
Sep 22, 2019·Nitric Oxide : Biology and Chemistry·Zs KolbertJ T Hancock
Mar 2, 2021·Frontiers in Plant Science·Zhen-Chao LiJian-Zhong Liu

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Methods Mentioned

BETA
ubiquitination
PCR
two-hybrid
reverse phase chromatography
pull-down
glycosylation

Software Mentioned

ImageJ
Geneious
Graphpad Prism
ProteoRed
PyMOL
MEGA7
ImageQuant

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