Regulation of secondary follicle growth by theca cells and insulin-like growth factor 1

The Journal of Reproduction and Development
Noriko Shiomi-SugayaAkira Iwase

Abstract

Ovaries contain follicles at various stages of development, including primordial, primary, secondary, antral and Graafian follicles. Although the growth of these follicles is controlled to maintain regular ovulation, the mechanism through which this occurs remains unclear. In our study, we found that the growth rate of cultured secondary follicles separated from mice ovaries differed between follicles. After 4 days of culture, the size of some secondary follicles was markedly increased, while that of others had either slightly increased, remained unchanged or shrunk. We compared the expression levels of growth factors between these secondary follicles and found that the growth rate of cultured secondary follicles correlated with the expression level of insulin-like growth factor 1 (Igf1) mRNA. Igf1 mRNA expression level in secondary follicles containing theca cells was higher than that in secondary follicles without theca cells, and the granulosa cell proliferation around follicles containing theca cells was increased. Furthermore, an IGF1 inhibitor also inhibited the granulosa cell proliferation, and administration of IGF1 to secondary follicles without growth promoted granulosa cell proliferation. These results indicated that...Continue Reading

References

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Citations

Mar 23, 2017·The Journal of Reproduction and Development·Kouji Komatsu, Satoru Masubuchi
Jul 13, 2019·Frontiers in Endocrinology·Carlos DosoutoPeter Humaidan
Dec 21, 2017·Reproductive Medicine and Biology·Kouji Komatsu, Satoru Masubuchi

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Methods Mentioned

BETA
confocal microscopy
PCR

Software Mentioned

ImageJ
R

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