Apr 10, 1976

Regulation of secretion from the adrenal medulla. Evidence for adenylate cyclase activity in secretory vesicle membranes

The Journal of Biological Chemistry
O ZinderH B Pollard


Adenylate cyclase activity has been found in purified secretory vesicle membranes from the adrenal medulla. Activity was detected both by formation of radioactive cAMP from [alpha-32P]ATP and by the competitive protein binding assay for cAMP. Activity was highest at pH 8.0 to 8.5, and was stimulated by sodium fluoride and GppNHp, a GTP analogue known to stimulate adenylate cyclase activity in plasma membrane preparations. The reaction rate was strongly dependent on the molar ratio of Mg2+:ATP in the system. This is the first demonstration of adenylate cyclase in a secretory vesicle membrane.

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Mentioned in this Paper

Tissue Membrane
Receptors, Drug
Plasma Protein Binding Capacity
Electron Microscopy
Cyclic AMP, (R)-Isomer
Bos indicus
Hydrogen-Ion Concentration
Adenylate Cyclase
Cytoplasmic Granules
Adrenal Medulla

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