Regulation of septum formation in Aspergillus nidulans by a DNA damage checkpoint pathway.

Genetics
Steven D Harris, Peter R Kraus

Abstract

In Aspergillus nidulans, germinating conidia undergo multiple rounds of nuclear division before the formation of the first septum. Previous characterization of temperature-sensitive sepB and sepJ mutations showed that although they block septation, they also cause moderate defects in chromosomal DNA metabolism. Results presented here demonstrate that a variety of other perturbations of chromosomal DNA metabolism also delay septum formation, suggesting that this is a general cellular response to the presence of sublethal DNA damage. Genetic evidence is provided that suggests that high levels of cyclin-dependent kinase (cdk) activity are required for septation in A. nidulans. Consistent with this notion, the inhibition of septum formation triggered by defects in chromosomal DNA metabolism depends upon Tyr-15 phosphorylation of the mitotic cdk p34nimX. Moreover, this response also requires elements of the DNA damage checkpoint pathway. A model is proposed that suggests that the DNA damage checkpoint response represents one of multiple sensory inputs that modulates p34nimX activity to control the timing of septum formation.

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Citations

Jan 15, 2017·Microbiology Spectrum·Ilkay Dörter, Michelle Momany
Apr 22, 2017·Microbiology Spectrum·Gero SteinbergSteven D Harris
Jul 9, 2005·Eukaryotic Cell·Marcia Regina von Zeska Kress FagundesGustavo Henrique Goldman
Aug 5, 2005·Genetics·Joel Fernandes LimaGustavo Henrique Goldman
Jun 7, 2003·Molecular Microbiology·Camile P SemighiniGustavo Henrique Goldman
Apr 4, 2002·Fungal Genetics and Biology : FG & B·Gustavo H GoldmanSteven D Harris
Mar 5, 2004·Fungal Genetics and Biology : FG & B·Gustavo H Goldman, Etta Kafer
Mar 17, 1999·FEMS Microbiology Reviews·R Fischer
May 23, 2002·FEMS Microbiology Letters·Peter R KrausSteven D Harris

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