Regulation of sialic acid 9-O-acetylation during the growth and differentiation of murine erythroleukemia cells.

The Journal of Biological Chemistry
W X ShiA Varki

Abstract

Sialic acids are typically found at the terminal position on vertebrate oligosaccharides. They are sometimes modified by an O-acetyl ester at the 9-position, potentially altering recognition of sialic acid by antibodies, lectins, and viruses. 9-O-Acetylation is known to be selectively expressed on gangliosides in melanoma cells and on N-linked chains in hepatocytes. Using a recently developed probe, we show here that in murine erythroleukemia cells, this modification is selectively expressed on another class of oligosaccharides, O-linked chains carried on cell surface sialomucins. These cells also express 9-O-acetylation on the ganglioside GD3, but this modification appears to be undetectable on the cell surface. Increasing cell density in culture is associated with a decrease in cell surface 9-O-acetylation of sialomucins. This change correlates with the spontaneous differentiation toward a mature erythroid phenotype. This down-regulation upon differentiation and entry into the G0/G1 stage of the cell cycle is confirmed by differentiation-inducing agents. In contrast, cells arrested in G2/M by the microtubule depolymerizing agent nocodazole show increased expression of cell surface 9-O-acetylated sialomucins (but not the 9-O-a...Continue Reading

References

Jan 1, 1978·Annual Review of Biochemistry·P A Marks, R A Rifkind
Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J S GoltzP Satir
Sep 30, 1992·Biochemical and Biophysical Research Communications·B KniepE P Rieber
Feb 1, 1992·Glycobiology·A Varki
Nov 1, 1991·The Journal of Cell Biology·J Tooze, M Hollinshead
Jan 1, 1990·Cell Motility and the Cytoskeleton·T E Kreis
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·A S Blum, C J Barnstable
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J B Ulmer, G E Palade
May 1, 1986·Biological Chemistry Hoppe-Seyler·A P CorfieldR Schauer
Feb 2, 1987·European Journal of Biochemistry·J P KamerlingJ F Vliegenthart
Feb 2, 1982·Biochimica Et Biophysica Acta·J P KamerlingM Wember
May 30, 1980·Biochemical and Biophysical Research Communications·G ReuterR J Howard
Jan 1, 1995·Methods in Enzymology·A Mellors, R Y Lo
Feb 17, 1995·The Journal of Biological Chemistry·E R SjobergA Varki
Jun 7, 1996·The Journal of Biological Chemistry·M J GuimarãesA Zlotnik
Jan 1, 1995·Annual Review of Cell and Developmental Biology·S J MorrisonI L Weissman

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May 20, 1998·Biochimie·A Klein, P Roussel
Oct 16, 2010·Glycobiology·Sigrid ArmingReinhard Vlasak
Apr 23, 2004·Biological Chemistry·Yanqin ShenRoland Schauer
Jun 23, 2015·Cell Reports·Martijn A LangereisRaoul J de Groot

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