PMID: 22332532Feb 16, 2012Paper

Regulation of sonic hedgehog on vascular endothelial growth factor, basic fibroblast growth factor expression and secretion in bone marrow mesenchymal stem cells

Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery
Jia-Qin CaiLi Deng

Abstract

Sonic hedgehog (Shh) signaling pathway is involved in an important part of regulating angiogenesis. To investigate the effects of recombinant Shh N-terminate (rShh-N) on the expression and secretion of angiogenesis-related factor-vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF). Bone marrow mesenchymal stem cells (BMSCs) were isolated from 3-day-old healthy Sprague Dawley rats and cultured to passage 3 in vitro. rShh-N at the concentrations of 0, 10, 100, and 200 ng/mL were applied to culture BMSCs in groups A, B, C, and D, respectively. At 12, 24, 48, and 72 hours of culture, the expressions of VEGF and bFGF mRNA and the levels of VEGF and bFGF in supernatant were measured with real-time quantitative PCR and ELISA, respectively. At the gene level, compared with group A, the expressions of VEGF and bFGF mRNA were enhanced in group D (P < 0.05) and the upregulation was more significant at 12 and 48 hours than 24 and 72 hours (P < 0.01). In group C, bFGF mRNA expression was substantially promoted at 12-72 hours (P < 0.05) and VEGF mRNA level was upregulated at 24-72 hours (P < 0.05), and both reached peak at 72 hours (P < 0.01). In group B, VEGF mRNA expression was inhibited at 12 hours (P < 0.0...Continue Reading

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