Regulation of spontaneous meiosis resumption in mouse oocytes by various conventional PKC isozymes depends on cellular compartmentalization

Journal of Cell Science
Nathalie AvazeriB Lefèvre

Abstract

In this study, we investigated the spatio-temporal distribution of conventional protein kinases C (cPKC) isoforms PKC-alpha, PKC-betaI, PKC-betaII and PKC-gamma in mouse oocytes. The cPKCs were present in the cytoplasm at the start of the process and migrated to the nucleus (or germinal vesicle) before germinal vesicle breakdown, except for PKC-gamma which remained cytoplasmic. In both compartments, the fully phosphorylated form corresponding to the 'mature' enzyme was revealed for PKC-alpha, PKC-betaI and PKC-betaII. Microinjection of specific antibodies against each isozyme in one or the other cell compartment at different times of the meiotic process, permitted us to observe the following: (1) When located in the cytoplasm at the beginning of the process, PKC-alpha is not implicated in germinal vesicle breakdown, PKC-betaI and PKC-gamma are involved in maintaining the meiotic arrest, and PKC-betaII plays a role in meiosis reinitiation. Furthermore, just before germinal vesicle breakdown, these cytoplasmic cPKCs were no longer implicated. (2) When located in the germinal vesicle, PKC-alpha, PKC-betaI and PKC-betaII are involved in meiosis reinitiation. Our data highlight not only the importance of the nuclear pathways in the ...Continue Reading

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Citations

Jan 15, 2011·Zygote : the Biology of Gametes and Early Embryos·Long-Bo CuiFang-Zhen Sun
Jan 1, 2009·Molecular Reproduction and Development·Stephen A Stricker
Jul 11, 2007·Molecular Reproduction and Development·F MiyaraB Lefèvre
Sep 25, 2009·Molecular Reproduction and Development·Madhavi KaliveDavid G Capco
Jul 24, 2015·Molecular Reproduction and Development·Wei MaMaria M Viveiros
Apr 1, 2006·Biochimica Et Biophysica Acta·Alberto M MartelliFrancesco Antonio Manzoli
Feb 8, 2019·Cell Cycle·Zi-Yun YiWei-Ping Qian

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