Regulation of sprouty expression by PLCgamma and calcium-dependent signals

Biochemical and Biophysical Research Communications
Makoto Abe, Michael C Naski

Abstract

Sprouty, an essential antagonist of fibroblast growth factor receptor signaling, is induced following fibroblast growth factor receptor activation. The signaling pathways that induce sprouty have been incompletely characterized. However, studies show that MAP kinase signaling stimulates sprouty induction in various cell lines. Here we report that activation of sprouty expression by basic fibroblast growth factor required phospholipase Cgamma (PLCgamma) and calcium-dependent signaling. We showed that the induction of sprouty was inhibited by chelation of intracellular or extracellular calcium and that a fibroblast growth factor receptor deficient for PLCgamma signaling only weakly induced sprouty expression. Additionally, inhibition of PLCgamma with a pharmacological antagonist repressed the induction of sprouty by basic fibroblast growth factor. These findings indicate that calcium-dependent signaling regulates sprouty expression and that PLCgamma is vital for this process. This pathway of sprouty induction may be critical at sites such as limb bud mesenchyme where MAP kinases are inactive.

References

Mar 25, 1999·Cell·T CasciM Freeman
May 20, 1999·Mechanisms of Development·A A de MaximyS Bellusci
Nov 1, 2000·Cell·J Schlessinger
Feb 13, 2001·Seminars in Cell & Developmental Biology·M D BootmanP Lipp
Aug 2, 2001·Biochemical and Biophysical Research Communications·K OzakiM Kohno
May 2, 2002·Proceedings of the National Academy of Sciences of the United States of America·James E EganDafna Bar-Sagi
Sep 20, 2002·The Journal of Biological Chemistry·Masuhiro TomitaMichael C Naski
Oct 29, 2002·Nature Cell Biology·Hiroshi HanafusaEisuke Nishida
Jan 16, 2003·Cell·Colin W Taylor
Feb 21, 2003·Current Biology : CB·Chanan RubinYosef Yarden
Feb 21, 2003·Current Biology : CB·Amy B HallDafna Bar-Sagi
Mar 22, 2003·Current Opinion in Cell Biology·Ivan Dikic, Silvia Giordano
May 27, 2003·Nature Cell Biology·Yasuhiko KawakamiJuan Carlos Izpisúa Belmonte
Jul 17, 2003·British Journal of Cancer·D C Sullivan, R Bicknell
Aug 20, 2003·Development·Laura Beth CorsonJanet Rossant
Oct 1, 2003·Angiogenesis·G Christofori
Apr 15, 2004·Science's STKE : Signal Transduction Knowledge Environment·Michael Tsang, Igor B Dawid

❮ Previous
Next ❯

Citations

Aug 20, 2010·Molecular Biology of the Cell·Simge AkbulutJonathan D Licht
Feb 3, 2006·Genes & Development·Sanjay JainJeffrey Milbrandt
Dec 3, 2008·Development, Growth & Differentiation·Makoto AbeSatoshi Wakisaka
Oct 9, 2015·Food & Nutrition Research·Salvador Fernández-ArroyoJesús Pintor
May 11, 2015·The International Journal of Biochemistry & Cell Biology·Angelina DoriguzziHedwig Sutterlüty-Fall
Dec 13, 2005·Trends in Cell Biology·Jacqueline M MasonJonathan D Licht

❮ Previous
Next ❯

Related Concepts

Related Feeds

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.