Regulation of stearoyl-CoA desaturase 1 mRNA stability by polyunsaturated fatty acids in 3T3-L1 adipocytes.

The Journal of Biological Chemistry
A M SesslerJames M Ntambi

Abstract

The effects of arachidonic acid (20:4, n-6) and other fatty acids on the expression of stearoyl-CoA desaturase gene 1 were investigated in fully differentiated 3T3-L1 adipocytes. Treatment of 3T3-L1 adipocytes with arachidonic acid resulted in a decrease in stearoyl-CoA desaturase (Scd) enzyme activity and scd1 mRNA. Arachidonic acid did not alter the transcription of the scd1 gene, whereas the half-life of the scd1 mRNA was reduced from 25.1 to 8.5 h. Blocking the conversion of arachidonic acid to eicosanoids by pretreatment of the cells with cyclooxygenase, lipoxygenase, or cytochrome P-450 epoxygenase inhibitors did not reverse the inhibition caused by arachidonic acid, indicating that eicosanoid synthesis is not necessary for the repression of scd1 mRNA expression. Treatment of adipocytes with linoleic (18:2, n-6) and linolenic (18:3, n-3) acids also resulted in inhibition of scd1 mRNA accumulation. By contrast, oleic acid (18:1, n-9) and stearic acid (18:0) had no effect on scd1 mRNA levels. Taken together, these results suggest that polyunsaturated fatty acids repress the expression of the scd1 gene in mature adipocytes by reducing the stability of scd1 mRNA.

References

Oct 1, 1991·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M T ClandininT R Clandinin
Jul 1, 1990·The British Journal of Nutrition·C J Simpson, A Wise
Apr 1, 1986·Archives of Biochemistry and Biophysics·L M Salati, S D Clarke
Sep 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·D A BernlohrT J Kelly
Jan 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·B C Reed, M D Lane
Jun 26, 1995·Biochemical and Biophysical Research Communications·J K Wolford, S A Signs
Jan 1, 1993·Progress in Lipid Research·S D Clarke, D B Jump
Apr 29, 1994·Biochemical and Biophysical Research Communications·K T LandschulzM D Lane
Jan 1, 1994·Annual Review of Nutrition·S D Clarke, D B Jump
Jan 1, 1994·Annual Review of Nutrition·P CorneliusM D Lane
Sep 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·D B JumpA Thelen

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Citations

Jan 6, 2012·Pflügers Archiv : European journal of physiology·Pallavi CheguruRodney A Hill
Apr 12, 2005·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·S L Hsieh, C-M Kuo
Sep 10, 2013·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Elizabeth BarberDavid Cameron-Smith
Jan 8, 1998·Biochimica Et Biophysica Acta·K M WatersJ M Ntambi
Dec 5, 2003·Progress in Lipid Research·James M Ntambi, Makoto Miyazaki
Nov 26, 1998·Progress in Lipid Research·D R TocherP A Hodgson
Dec 22, 1999·The Proceedings of the Nutrition Society·T Raclot, H Oudart
Oct 7, 2005·Biology of Reproduction·Céline MoreauJoëlle Dupont
Oct 11, 2005·Annual Review of Cell and Developmental Biology·Joseph R Marszalek, Harvey F Lodish
Jul 14, 2005·Annual Review of Nutrition·Harini Sampath, James M Ntambi
Jun 8, 2010·Lipids in Health and Disease·Elizabeth ManickamDavid Cameron-Smith
Nov 10, 2010·Molecular Medicine·Sara García-SerranoEduardo García-Fuentes
Dec 17, 2009·Redox Report : Communications in Free Radical Research·Yorihiro YamamotoMasahiko Tanaka
Mar 21, 1998·Insect Biochemistry and Molecular Biology·C Wicker-ThomasR Dallerac
Oct 23, 2004·Nutrition Reviews·Harini Sampath, James M Ntambi
Dec 21, 2002·European Journal of Biochemistry·Tao ChenThomas H MacRae
Feb 13, 2015·International Journal of Molecular Sciences·Zuzana Papackova, Monika Cahova
Sep 25, 2012·Progress in Lipid Research·Leanne Hodson, Barbara A Fielding
Jul 4, 2012·The Journal of Nutritional Biochemistry·Ana Maria LottenbergEdna Regina Nakandakare
May 23, 2013·Clinical Physiology and Functional Imaging·Fatma J Al-Saeedi, Behling Cheng
Dec 1, 2010·Journal of Animal Physiology and Animal Nutrition·I J KarlengenO Taugbøl
Aug 21, 2015·Journal of Animal Science and Technology·Crystal M WarnerHyungchul Han
Nov 29, 2005·Best Practice & Research. Clinical Endocrinology & Metabolism·Hadi Al-Hasani, Hans-Georg Joost
May 12, 2015·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Jessica C Ralston, David M Mutch
Jul 1, 1999·Toxicology and Applied Pharmacology·M ShinC Umezawa

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