Regulation of steroid hydroxylase CYP7B1 by androgens and estrogens in prostate cancer LNCaP cells

Biochemical and Biophysical Research Communications
Wanjin Tang, M Norlin

Abstract

The present study reports effects of androgens and estrogens on human CYP7B1 transcription in prostate cancer LNCaP cells. Studies with rodents have suggested a role for the CYP7B1 enzyme in balancing cellular hormone levels important for prostate growth. Little is, however, known about the regulation of human CYP7B1. The current study showed strong suppression of a human CYP7B1 luciferase reporter gene by dihydrotestosterone (DHT) in prostate cancer LNCaP cells. Also, DHT and overexpression of androgen receptor (AR) suppressed CYP7B1 promoter activity and CYP7B1-mediated catalysis in kidney-derived HEK293 cells. Effects on CYP7B1 transcription were observed also by estrogen receptors (ER). The effects appeared different for different estrogens. CYP7B1 was stimulated by synthetic ER agonists but suppressed by 17beta-estradiol and 5alpha-androstane-3beta,17beta-diol in LNCaP cells. Our data indicate an important role for CYP7B1 in balancing prostate hormone levels in human cells. In particular, the data suggest that androgens may control intraprostatic levels of estrogen via regulation of CYP7B1-mediated metabolism.

References

Nov 1, 1986·Journal of Steroid Biochemistry·K D Voigt, W Bartsch
May 13, 1997·Proceedings of the National Academy of Sciences of the United States of America·K A RoseR Lathe
Jul 27, 1999·The Journal of Steroid Biochemistry and Molecular Biology·A O BrinkmannJ Trapman
Jul 30, 1999·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·K J CudeW D Figg
Apr 5, 2000·The Journal of Biological Chemistry·J Li-HawkinsD W Russell
Jul 7, 2000·The Journal of Biological Chemistry·M NorlinK Wikvall
Oct 9, 2002·Proceedings of the National Academy of Sciences of the United States of America·Zhang WeihuaJan-Ake Gustafsson
May 22, 2003·Metabolism: Clinical and Experimental·Shunlin RenWilliam M Pandak
Jun 7, 2003·Endocrine-related Cancer·G P RisbridgerS J McPherson
Mar 9, 2004·Biochemical and Biophysical Research Communications·Maria Norlin, John Y L Chiang
Jun 8, 2004·The Journal of Clinical Endocrinology and Metabolism·Cécile MartinFouad K Habib
Jan 25, 2005·The Journal of Steroid Biochemistry and Molecular Biology·Gerald R CunhaTakeshi Kurita
Jan 25, 2005·The Journal of Steroid Biochemistry and Molecular Biology·Pirkko L Härkönen, Sari I Mäkelä
Feb 16, 2005·Proceedings of the National Academy of Sciences of the United States of America·Yoko OmotoJan-Ake Gustafsson
May 25, 2006·The Journal of Steroid Biochemistry and Molecular Biology·Wanjin TangMaria Norlin

❮ Previous
Next ❯

Citations

Feb 5, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Carlos PenalozaZahra Zakeri
Sep 5, 2009·The Journal of Clinical Investigation·Nicolas LeuenbergerWalter Wahli
Nov 14, 2015·The Journal of Steroid Biochemistry and Molecular Biology·Michihisa Umetani
Jan 4, 2007·The Journal of Pathology·N SampsonP Berger
Nov 30, 2019·Biology of Sex Differences·Taylor PhelpsPamela Harvey
Mar 28, 2021·Proceedings of the National Academy of Sciences of the United States of America·Wan-Fu WuJan-Ake Gustafsson
Jul 23, 2021·Liver Research·John Y L Chiang, Jessica M Ferrell
May 8, 2007·Archives of Biochemistry and Biophysics·Wanjin TangKjell Wikvall

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.