PMID: 9523531Apr 2, 1998Paper

Regulation of stimulated cyclic AMP synthesis by urocanic acid

Photochemistry and Photobiology
B BouscarelE C DeFabo

Abstract

Urocanic acid (UCA) has been shown to mediate the UVB radiation-induced immunosuppression initiated in the skin by UV-induced isomerization from the trans to the cis isomer. However, the mechanism by which cis-UCA acts is still unclear. Therefore, the present study was undertaken to determine the effect of trans- and cis-UCA on cyclic adenosine 3',5'-monophosphate (cAMP) synthesis in human dermal fibroblasts, Golden Syrian hamster hepatocytes and in the human adenocarcinoma cell line, HT29. Neither trans- nor cis-UCA was able to stimulate cAMP synthesis directly in any of the models tested. In human dermal fibroblasts, cis-UCA, in contrast to trans-UCA, specifically inhibited cAMP synthesis induced by either prostaglandin (PG) E1 or PGE2 with a maximum inhibitory effect of 25-30% at cis-UCA concentrations greater than 1 microM and half-maximum inhibitory effect (EC50) observed at 35 nM. The effect of cis-UCA was not to stimulate phosphodiesterase and cAMP breakdown. The inhibitory effect of cis-UCA (an imidazole derivative) was not mediated through stimulation of the alpha 2-adrenergic receptor. The inhibitory effect of cis-UCA on stimulated cAMP synthesis was a function of the cell density and was only significant when the fib...Continue Reading

Related Concepts

Related Feeds

Adrenergic Receptors: Trafficking

Adrenergic receptor trafficking is an active physiological process where adrenergic receptors are relocated from one region of the cell to another or from one type of cell to another. Discover the latest research on adrenergic receptor trafficking here.

Related Papers

European Journal of Dermatology : EJD
Zekayi KutlubayIlkin Zindanci
The Journal of Investigative Dermatology
Vilija OkeMarie Wahren-Herlenius
Journal of Clinical Rheumatology : Practical Reports on Rheumatic & Musculoskeletal Diseases
Shamael Alsanafi, Victoria P Werth
The Journal of Immunology : Official Journal of the American Association of Immunologists
Atsushi FukunagaStephen E Ullrich
© 2022 Meta ULC. All rights reserved