Regulation of store-operated Ca2+ entry activity by cell cycle dependent up-regulation of Orai2 in brain capillary endothelial cells

Biochemical and Biophysical Research Communications
Hiroaki KitoYuji Imaizumi

Abstract

Store-operated Ca(2+) entry (SOCE) via Orai1 and STIM1 complex is supposed to have obligatory roles in the regulation of cellular functions of vascular endothelial cells, while little is known about the contribution of Orai2. Quantitative PCR and Western blot analyses indicated the expression of Orai2 and STIM2, in addition to Orai1 and STIM1 in bovine brain capillary endothelial cell line, t-BBEC117. During the exponential growth of t-BBEC117, the knockdown of Orai1 and STIM1 significantly reduced the SOCE activity, whereas Orai2 and STIM2 siRNAs had no effect. To examine whether endogenous SOCE activity contributes to the regulation of cell cycle progression, t-BBEC117 were synchronized using double thymidine blockage. At the G2/M phase, Ca(2+) influx via SOCE was decreased and Orai2 expression was increased compared to the G0/G1 phase. When Orai2 was knocked down at the G2/M phase, the decrease in SOCE was removed, and cell proliferation was partly attenuated. Taken together, Orai1 significantly contributes to cell proliferation via the functional expression, which is presumably independent of the cell cycle phases. In construct, Orai2 is specifically up-regulated during the G2/M phase, negatively modulates the SOCE activity...Continue Reading

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Citations

Apr 19, 2016·Brain Research·Nurul Adhwa RahmanSiti Hanna Muharram
May 29, 2016·Biochemical and Biophysical Research Communications·Hideto YamamuraYuji Imaizumi
Aug 17, 2019·International Journal of Molecular Sciences·Francesco MocciaGermano Guerra
Jan 13, 2018·International Journal of Molecular Sciences·Francesco Moccia
Nov 5, 2017·American Journal of Physiology. Heart and Circulatory Physiology·Bojun ZhangThomas C Resta

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