Apr 4, 2006

Regulation of stretch-induced JNK activation by stress fiber orientation

Cellular Signalling
Roland KaunasShu Chien

Abstract

Cyclic mechanical stretch associated with pulsatile blood pressure can modulate cytoskeletal remodeling and intracellular signaling in vascular endothelial cells. The aim of this study was to evaluate the role of stretch-induced actin stress fiber orientation in intracellular signaling involving the activation of c-jun N-terminal kinase (JNK) in bovine aortic endothelial cells. A stretch device was designed with the capability of applying cyclic uniaxial and equibiaxial stretches to cultured endothelial cells, as well as changing the direction of cyclic uniaxial stretch. In response to 10% cyclic equibiaxial stretch, which did not result in stress fiber orientation, JNK activation was elevated for up to 6 h. In response to 10% cyclic uniaxial stretch, JNK activity was only transiently elevated, followed by a return to basal level as the actin stress fibers became oriented perpendicular to the direction of stretch. After the stress fibers had aligned perpendicularly and the JNK activity had subsided, a 90-degree change in the direction of cyclic uniaxial stretch reactivated JNK, and this activation again subsided as stress fibers became re-oriented perpendicular to the new direction of stretch. Disrupting actin filaments with cy...Continue Reading

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  • Citations59

Citations

Mentioned in this Paper

Pathologic Cytolysis
Neuro-Oncological Ventral Antigen 2
JUN gene
JUND gene
Stress, Mechanical
Dimethyl Sulfoxide
MAPK8 gene
Vascular Endothelial Cells
Actins
SDS-PAGE

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