Regulation of subtilisin-like protease prC expression by nematode cuticle in the nematophagous fungus Clonostachys rosea

Environmental Microbiology
Chenggang ZouKeqin Zhang

Abstract

Nematophagous fungi have been used as biological control agents against nematodes parasitic to plants and animals. These fungi can secret subtilisin-like extracellular serine proteases during the infection of nematodes. The expression of these subtilisin-like serine proteases is regulated by nitrogen sources, including nematode cuticle. However, the mechanisms underlying the nitrogen sources-induced expression of these serine proteases is not well understood. In this study, we investigated the effect of nitrogen sources on the expression of a subtilisin-like extracellular protease, prC, in the nematophagous fungus Clonostachys rosea. Disruption of prC attenuated infection of the fungus to nematodes, indicating that this gene functions as a virulence factor. The inhibition of basal expression of prC by the preferred nitrogen sources (glutamine, ammonia) occurred at the transcriptional level. In contrast, nematode cuticle induced the expression of prC at the post-transcriptional level. The inducible expression of prC by nematode cuticle was significantly suppressed by glutamine, ammonia and phenylmethylsulfonyl fluoride (an inhibitor of serine protease). Thus, the existence of active PrC, albeit at a low level in the medium, is p...Continue Reading

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Citations

Jul 9, 2013·Applied Microbiology and Biotechnology·Jinkui YangKe-Qin Zhang
Oct 8, 2013·Biotechnology Advances·Sina Adrangi, Mohammad Ali Faramarzi
Jan 10, 2018·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Ruizhen WangYong-An Zhang
Mar 3, 2020·Journal of Applied Microbiology·Z-B SunM-H Sun
Jul 28, 2020·Applied and Environmental Microbiology·Yu-Zhong ZhangXiu-Lan Chen
Mar 11, 2017·Microbiology Spectrum·Magnus KarlssonSusanne Zeilinger

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