Regulation of SUMO2 target proteins by the proteasome in human cells exposed to replication stress

Journal of Proteome Research
Sara BursomannoYing Liu

Abstract

In human cells, SUMO2 is predominantly conjugated to target proteins in response to cellular stress. Previous studies suggested that proteins conjugated to SUMO2, but not to SUMO1, could be regulated by the ubiquitin-mediated proteasome system. Hence, we set out to understand the role of the proteasome in determining the fate of proteins conjugated to SUMO2 when cells are treated with DNA replication stress conditions. We conducted a quantitative proteomic analysis in a U2OS cell line stably expressing SUMO2(Q87R) tagged with StrepHA in the presence or absence of epoxomicin (EPOX), a proteasome inhibitor. We identified subgroups of putative SUMO2 targets that were either degraded or stabilized by EPOX upon SUMO2 conjugation in response to replication stress. Interestingly, the subgroup of proteins degraded upon SUMO2 conjugation was enriched in proteins playing roles in DNA damage repair and replication, while the proteins stabilized upon SUMOylation were mainly involved in chromatin maintenance. In addition, we identified 43 SUMOylation sites in target proteins, of which 17 are located in the proximity of phosphorylated residues. Considering that DNA replication stress is a major source of genome instability, which is suggeste...Continue Reading

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Citations

Mar 8, 2016·Nature Structural & Molecular Biology·Emilio LeconaOscar Fernandez-Capetillo
May 18, 2016·Nucleic Acids Research·Kouji HirotaShunichi Takeda
Jul 21, 2016·Nature Reviews. Molecular Cell Biology·Ivo A Hendriks, Alfred C O Vertegaal
Sep 27, 2016·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Emilio Lecona, Oscar Fernandez-Capetillo

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