Regulation of syndecan-4 phosphorylation in vivo.

The Journal of Biological Chemistry
Arie Horowitz, Michael Simons

Abstract

Recent studies suggest that some of the heparan sulfate-carrying proteoglycans may directly participate in signaling via their cytoplasmic tail. The present investigation addresses the potential involvement of syndecan-4, a widely expressed transmembrane proteoglycan, in this process. We found that the cytoplasmic tail of syndecan-4 is phosphorylated on a single serine residue (Ser183) in growth-arrested NIH 3T3 fibroblasts, with a stoichiometry of 0.3 mol Pi/mol syndecan-4. Treatment of the cells with a protein kinase C (PKC)-activating phorbol ester lead to a 2.5-fold increase in Ser183 phosphorylation. This increase was inhibited by a generic PKC inhibitor but not by an inhibitor specific to the calcium-dependent conventional PKCs, suggesting that the cytoplasmic tail of syndecan-4 is phosphorylated by a calcium-independent novel PKC isozyme. Application of 10-30 ng/ml basic fibroblast growth factor (bFGF) produced a 2-3-fold reduction in the phosphorylation of syndecan-4. Because treatment with the phosphatase inhibitor calyculin prevented the bFGF-induced decrease in syndecan-4 phosphorylation, the effect of bFGF appears to be mediated by a protein serine/threonine phosphatase type 1 or 2A. We conclude that the cytoplasmic...Continue Reading

References

Jan 1, 1992·Annual Review of Cell Biology·M BernfieldE J Lose
Apr 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S E GouldR A Kosher
Nov 15, 1990·Biochemical and Biophysical Research Communications·J M HerbertJ P Maffrand
Apr 1, 1989·The Journal of Cell Biology·S SaundersM Bernfield
Jan 1, 1994·Annual Review of Cell Biology·P van der GeerR A Lindberg
Feb 15, 1993·Biochemical and Biophysical Research Communications·T KojimaH Saito
Oct 1, 1993·Current Opinion in Cell Biology·A C Rapraeger
Nov 1, 1995·Molecular Biology of the Cell·P C Baciu, P F Goetinck
Jan 10, 1997·The Journal of Biological Chemistry·K NishikawaL C Cantley
Mar 28, 1997·The Journal of Biological Chemistry·E S OhJ R Couchman
Aug 1, 1997·Archives of Biochemistry and Biophysics·E S OhA Woods

❮ Previous
Next ❯

Citations

Feb 23, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·S Jaken, P J Parker
Dec 18, 2001·Medicinal Research Reviews·Jian Liu, Suzanne C Thorp
Mar 1, 1994·Trends in Biochemical Sciences·J J BergeronD B Williams
Dec 1, 2001·Cellular Signalling·M Simons, A Horowitz
Jan 9, 1999·Matrix Biology : Journal of the International Society for Matrix Biology·A WoodsJ R Couchman
Sep 7, 2001·Current Opinion in Cell Biology·A Woods, J R Couchman
Nov 18, 1998·Current Opinion in Cell Biology·A C Rapraeger, V L Ott
Mar 15, 2000·The International Journal of Biochemistry & Cell Biology·S TumovaJ R Couchman
Feb 25, 2000·The International Journal of Biochemistry & Cell Biology·M A Nugent, R V Iozzo
Feb 21, 2009·The Journal of Biological Chemistry·Béatrice SulkaPatricia Rousselle
Jun 29, 2000·Annual Review of Biochemistry·M BernfieldM Zako
Apr 27, 2011·Cardiovascular Diabetology·Célia M C StrunzMonica V Marquezini
Jan 9, 2004·Reproductive Biology and Endocrinology : RB&E·DeannaLee M Beauvais, Alan C Rapraeger
Mar 17, 2010·Cellular and Molecular Life Sciences : CMLS·Aniko Keller-PinterLaszlo Szilak
May 16, 2002·The Journal of Cell Biology·Arie HorowitzMichael Simons
Nov 26, 2011·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Yan SongSandra G Velleman
Sep 24, 2011·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Yan SongSandra G Velleman
Sep 16, 2010·The FEBS Journal·Tina Manon-JensenJohn R Couchman
Mar 8, 2005·Journal of Theoretical Biology·Kimberly Forsten-WilliamsMatthew A Nugent
Dec 15, 2004·Experimental and Molecular Pathology·Katherine D Bortoff, William D Wagner
Nov 29, 2005·Journal of Molecular Biology·Bon-Kyung KooWeontae Lee
Aug 31, 2000·Experimental Cell Research·C S LebakkenA C Rapraeger
May 1, 1999·Experimental Cell Research·F GranésS Vilaró
Nov 14, 2006·The International Journal of Biochemistry & Cell Biology·Annika N AlexopoulouJohn R Couchman
Apr 9, 2001·Seminars in Cell & Developmental Biology·A C Rapraeger
May 18, 1999·The Journal of Biological Chemistry·Y ZhangM Simons
Jul 27, 2000·Journal of Cellular Physiology·Y GaoM Simons
Dec 20, 2003·Nature Reviews. Molecular Cell Biology·John R Couchman
Jul 16, 2003·FEBS Letters·Francesc GranésSenén Vilaró
Jan 15, 2002·Arteriosclerosis, Thrombosis, and Vascular Biology·Lei Li, Elliot L Chaikof
May 6, 2006·Circulation Research·Eugene TkachenkoMichael Simons
Mar 19, 2005·Circulation Research·Eugene TkachenkoMichael Simons
Apr 24, 2009·Natural Product Reports·Sherket PetersonJian Liu
Oct 29, 2005·The Journal of Biological Chemistry·Sungmun ChoiEok-Soo Oh
Jun 28, 2002·The Journal of Biological Chemistry·Sarah A Wilcox-AdelmanPaul F Goetinck
Jun 5, 2012·Molecular Biology Reports·Yan SongSandra G Velleman

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.