PMID: 2500022Jun 1, 1989Paper

Regulation of synthesis of prostacyclin and HETEs in human endothelial cells

The American Journal of Physiology
B O IbeW B Campbell

Abstract

Human umbilical endothelial cells in culture synthesize prostacyclin (PGI2), 15-hydroxyeicosatetraenoic acid (15-HETE), and 12-hydroxyeicosatetraenoic acid (12-HETE). The synthesis of these eicosanoids was measured by specific radioimmunoassays after stimulation by arachidonic acid, A23187, bradykinin, melittin, or histamine. Under all conditions, the synthesis of PGI2 paralleled and exceeded the synthesis of 15-HETE and 12-HETE. Indomethacin inhibited arachidonic acid-stimulated PGI2 and 15-HETE synthesis but enhanced 12-HETE synthesis. Meclofenamate gave similar qualitative results. Drugs that act as inhibitors of lipoxygenase in some tissues, such as nordihydroguaiaretic acid (NDGA), caffeic acid, esculin, diethylcarbamazine, quercetin, and 5,8,11,14-eicosatetrayenoic acid (ETYA) were nonspecific in their inhibition of PGI2, 12-HETE, and 15-HETE synthesis. For example, NDGA inhibited arachidonic acid-stimulated release with a 50% inhibitory concentration (IC50) of 0.39 microM for PGI2, 0.25 microM for 15-HETE, and 0.10 microM for 12-HETE. These results show that endothelial cells metabolize both endogenous and exogenous arachidonic acid to PGI2, 15-HETE, and 12-HETE. These data also suggest, based on results with inhibitors,...Continue Reading

References

May 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·P Borgeat, B Samuelsson
Sep 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·M Hamberg, B Samuelsson
Aug 14, 1980·Biochemical and Biophysical Research Communications·M Hamberg, G Hamberg
Aug 1, 1982·Prostaglandins·K M Mullane, S Moncada
Apr 15, 1983·Biochemical and Biophysical Research Communications·J LarrueH Bricaud
Feb 27, 1980·Biochemical and Biophysical Research Communications·N L BaenzigerP R Becherer

❮ Previous
Next ❯

Citations

Jul 1, 1996·European Journal of Immunology·K M StuhlmeierF H Bach
Jun 1, 1993·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·R M WeisbrodR A Cohen
Jul 1, 1993·Cell and Tissue Research·L J Andries, D L Brutsaert
Feb 4, 1999·Endothelium : Journal of Endothelial Cell Research·J A HollandL M Ziegler
Aug 14, 1999·Japanese Journal of Pharmacology·S TakaiM Miyazaki
Jun 21, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Muthuvel JayachandranVirginia M Miller
Jan 1, 1991·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·M L Brown, D Deykin
Apr 1, 1990·Journal of Cellular Physiology·J A HollandM B Stemerman
Jun 24, 2000·Endothelium : Journal of Endothelial Cell Research·J A HollandL M Ziegler
Jan 1, 1997·Endothelium : Journal of Endothelial Cell Research·J A HollandL F Lemanski
Apr 15, 2014·European Journal of Pharmacology·Christina Alves Peixoto, Bruna Santos Silva
Jul 29, 1998·Free Radical Biology & Medicine·M MoutetJ Chaudière
Feb 24, 1991·European Journal of Pharmacology·M RosolowskyW B Campbell
Mar 1, 1994·Infection and Immunity·S G FillerJ E Edwards
Sep 29, 1993·Biochimica Et Biophysica Acta·S LópezC de Castellarnau
Jan 1, 1990·Progress in Lipid Research·R E WhatleyS M Prescott

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anthelmintics (ASM)

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.

Anthelmintics

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.