Regulation of TGF-beta 1-induced connective tissue growth factor expression in airway smooth muscle cells

American Journal of Physiology. Lung Cellular and Molecular Physiology
Shaoping XieKian Fan Chung

Abstract

Transforming growth factor (TGF)-beta may play an important role in airway remodeling, and the fibrogenic effect of TGF-beta may be mediated through connective tissue growth factor (CTGF) release. We investigated the role of MAPKs and phosphatidylinositol 3-kinase (PI3K) and the effects of inflammatory cytokines on TGF-beta-induced CTGF expression in human airway smooth muscle cells (ASMC). We examined whether Smad signal was involved in the regulatory mechanisms. TGF-beta 1 induced a time- and concentration-dependent expression of CTGF gene and protein as analyzed by real-time RT-PCR and Western blot. Inhibition of ERK and c-jun NH(2)-terminal kinase (JNK), but not of p38 MAPK and PI3K, blocked the effect of TGF-beta 1 on CTGF mRNA and protein expression and on Smad2/3 phosphorylation. T helper lymphocyte 2-derived cytokines, IL-4 and IL-13, attenuated TGF-beta 1-stimulated mRNA and protein expression of CTGF and inhibited TGF-beta 1-stimulated ERK1/2 and Smad2/3 activation in ASMC. The proinflammatory cytokines tumor necrosis factor-alpha and IL-1 beta reduced TGF-beta 1-stimulated mRNA expression of CTGF but did not inhibit TGF-beta-induced Smad2/3 phosphorylation. TGF-beta 1-stimulated CTGF expression is mediated by mechani...Continue Reading

References

Aug 1, 1997·American Journal of Respiratory and Critical Care Medicine·A M VignolaJ Bousquet
Aug 1, 1997·American Journal of Respiratory and Critical Care Medicine·A E RedingtonP H Howarth
Oct 6, 1997·American Journal of Respiratory Cell and Molecular Biology·E M MinshallQ Hamid
Feb 3, 1998·American Journal of Respiratory Cell and Molecular Biology·M JohnK F Chung
Feb 14, 1998·Cytokine & Growth Factor Reviews·G R Grotendorst
Jun 17, 1998·Genes & Development·M P de CaesteckerR J Lechleider
Sep 5, 1998·The American Journal of Physiology·K M HawkerJ L Black
Dec 16, 1998·American Journal of Respiratory and Critical Care Medicine·H W ChuS E Wenzel
Aug 24, 1999·Thorax·K F Chung, P J Barnes
Dec 22, 1999·The Journal of Biological Chemistry·M E EngelH L Moses
Mar 11, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·P CohenD J Herrick
Jun 1, 2000·Gene·C M Zimmerman, R W Padgett
Sep 1, 2000·The European Respiratory Journal·J G MartinD H Eidelman
Jan 21, 2001·The Journal of Biological Chemistry·A HolmesA Leask
Apr 6, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·A CouttsN Khalil
Jun 8, 2001·Proceedings of the National Academy of Sciences of the United States of America·J ZavadilE P Bottinger
Sep 29, 2001·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·H YokoiK Nakao
Apr 23, 2002·Journal of Cellular Biochemistry·David C RishikofRonald H Goldstein
Jul 23, 2002·Nature Cell Biology·José G AbreuE M De Robertis
Sep 18, 2002·Kidney International·Youjun ChenAndrew Leask
Dec 28, 2002·American Journal of Respiratory and Critical Care Medicine·Janette K BurgessJudith L Black
Feb 13, 2003·Reproduction : the Official Journal of the Society for the Study of Fertility·D K BallD R Brigstock
May 23, 2003·American Journal of Respiratory Cell and Molecular Biology·Mitsuyoshi UtsugiMasatomo Mori
Jun 18, 2003·Cell·Yigong Shi, Joan Massagué
Mar 9, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Gary R GrotendorstMatthew R Duncan

❮ Previous
Next ❯

Citations

Jan 17, 2014·Nature Communications·E Josue RuizJuan-Jose Ventura
Jan 13, 2006·Clinical and Experimental Pharmacology & Physiology·Janette K Burgess
May 1, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Shaoping XieKian Fan Chung
Aug 25, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Weichao GuoJoseph A Lasky
Dec 7, 2010·American Journal of Physiology. Lung Cellular and Molecular Physiology·Charalambos MichaeloudesKian Fan Chung
Sep 24, 2005·American Journal of Respiratory and Critical Care Medicine·Peter R A JohnsonJudith L Black
Jul 30, 2011·American Journal of Respiratory and Critical Care Medicine·Charalambos MichaeloudesKian Fan Chung
Jan 2, 2007·American Journal of Respiratory Cell and Molecular Biology·Shu WuEduardo Bancalari
Oct 7, 2008·American Journal of Respiratory Cell and Molecular Biology·John F AlcornYvonne M W Janssen-Heininger
Sep 29, 2009·American Journal of Respiratory Cell and Molecular Biology·Philip R CooperR Graham Sturton
Mar 13, 2008·Journal of Cell Science·John F AlcornYvonne M W Janssen-Heininger
Nov 24, 2005·Clinical Medicine & Research·Chrishan S Samuel
Mar 25, 2009·Experimental & Molecular Medicine·Misa NakamuraKennichi Kakudo
Apr 25, 2009·Journal of Cell Communication and Signaling·Jana SamarinMargarete Goppelt-Struebe
Aug 19, 2007·Journal of Internal Medicine·Kjell Larsson
Aug 30, 2014·Journal of Molecular and Cellular Cardiology·Kristin V T EngebretsenTheis Tønnessen
Apr 10, 2007·The Journal of Allergy and Clinical Immunology·Xiuxia ZhouSally E Wenzel
Mar 25, 2014·Pulmonary Pharmacology & Therapeutics·Hatem AlkhouriMichael Schuliga
Feb 23, 2017·Pharmacology & Therapeutics·MirHojjat KhorasanizadehNima Rezaei
Jul 28, 2005·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Valérie HaydontMarie-Catherine Vozenin-Brotons
Aug 19, 2007·Cancer Genetics and Cytogenetics·Sandra KarlssonKarin Klinga Levan
Jan 13, 2009·Pulmonary Pharmacology & Therapeutics·Janette K BurgessJane Ward
Oct 1, 2007·Expert Review of Respiratory Medicine·Kian Fan Chung
Jun 14, 2016·Molecular Cell·Dafni-Eleftheria PefaniEric O' Neill
May 16, 2006·Journal of the Society for Gynecologic Investigation·Marianna De FalcoAndrea Di Lieto
May 1, 2010·Journal of Cellular Physiology·X ZhangS N Popoff
Dec 25, 2015·Circulation Research·Peng ZhangXiao Yang
Jun 1, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jenny H KimEllen Puré
Sep 9, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Christian Bronner

❮ Previous
Next ❯

Related Concepts

Related Feeds

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.