Regulation of TGFbeta1-mediated collagen formation by LOX-1: studies based on forced overexpression of TGFbeta1 in wild-type and lox-1 knock-out mouse cardiac fibroblasts.

The Journal of Biological Chemistry
Chang Ping HuJawahar L Mehta

Abstract

Transforming growth factor beta(1) (TGFbeta(1)) activation leads to tissue fibrosis. Here, we report on the role of LOX-1, a lectin-like 52-kDa receptor for oxidized low density lipoprotein, in TGFbeta(1)-mediated collagen expression and underlying signaling in mouse cardiac fibroblasts. TGFbeta(1) was overexpressed in wild-type (WT) and LOX-1 knock-out mouse cardiac fibroblasts by transfection with adeno-associated virus type 2 vector carrying the active TGFbeta(1) moiety (AAV/TGFbeta (ACT)(1)). Transfection of WT mouse cardiac fibroblasts with AAV/TGFbeta (ACT)(1) markedly enhanced the expression of NADPH oxidases (p22(phox), p47(phox), and gp91(phox) subunits) and LOX-1, formation of reactive oxygen species, and collagen synthesis, concomitant with an increase in the activation of p38 and p44/42 mitogen-activated protein kinases (MAPK). The TGFbeta(1)-mediated increase in collagen synthesis was markedly attenuated in the LOX-1 knock-out mouse cardiac fibroblasts as well as in WT mouse cardiac fibroblasts treated with a specific anti-LOX-1 antibody. Treatment with anti-LOX-1 antibody also reduced NADPH oxidase expression and MAPK activation. The NADPH oxidase inhibitors and gp91phox small interfering RNA reduced LOX-1 express...Continue Reading

References

May 23, 1998·Annual Review of Immunology·J J Letterio, A B Roberts
May 4, 2000·The New England Journal of Medicine·G C BlobeH F Lodish
Jun 2, 2000·Biochemical and Biophysical Research Communications·M MinamiT Kita
Jun 17, 2003·Journal of Cardiovascular Pharmacology and Therapeutics·Jacob JosephJawahar L Mehta
Jul 8, 2003·The Journal of Pathology·Sylvia HeenemanMat J Daemen
Feb 14, 2006·American Journal of Physiology. Cell Physiology·Kensuke NomaJames K Liao
Apr 25, 2006·Biochemical and Biophysical Research Communications·Dayuan LiJawahar L Mehta
Mar 29, 2007·Cardiovascular Research·Jawahar L Mehta, Håvard Attramadal
May 5, 2007·Circulation Research·Jawahar L MehtaTatsuya Sawamura
Jun 15, 2007·The Journal of Biological Chemistry·Peter V UsatyukViswanathan Natarajan
Aug 21, 2007·Cardiovascular Research·Changping HuJawahar L Mehta
Aug 25, 2007·Arteriosclerosis, Thrombosis, and Vascular Biology·Abhijit DandapatJawahar L Mehta
Oct 26, 2007·American Journal of Physiology. Cell Physiology·Wenhui HuKarnam S Murthy

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Citations

Aug 24, 2011·Cardiovascular Drugs and Therapy·Xianwei WangJawahar L Mehta
Sep 14, 2011·Cardiovascular Drugs and Therapy·Ping LuoChangping Hu
Sep 29, 2011·Cardiovascular Drugs and Therapy·Sona MitraJawahar L Mehta
Jan 31, 2012·Current Atherosclerosis Reports·Tanu GoyalJawahar L Mehta
Nov 19, 2010·The Journal of Biological Chemistry·Chad CsepeggiAndrey Frolov
Aug 19, 2009·Journal of Cardiovascular Pharmacology·Bum-Yong KangJawahar L Mehta
Mar 25, 2014·Journal of Translational Medicine·Nicolas ChatauretThierry Hauet
Nov 6, 2012·Cellular and Molecular Life Sciences : CMLS·Suowen XuPeiqing Liu
Jul 27, 2010·Heart Failure Reviews·Adam NabeebaccusAjay M Shah
May 10, 2015·Chinese Journal of Integrative Medicine·Yan-fang XuZheng Zhang
Dec 30, 2014·Critical Reviews in Clinical Laboratory Sciences·Andreja TrpkovicEsma R Isenovic
Aug 15, 2014·Radiation Research·Eun Joo ChungDeborah Citrin
Dec 17, 2014·PloS One·Takeru ShirakiShinya Minatoguchi
Nov 10, 2012·American Heart Journal·Chiara MelloniL Kristin Newby
Mar 6, 2017·Life Sciences·Tian-Tian ZhuChang-Ping Hu
Oct 14, 2010·Journal of Cardiovascular Pharmacology and Therapeutics·Magomed KhaidakovJawahar L Mehta
May 9, 2018·Current Medicinal Chemistry·Ajoe John KattoorJawahar L Mehta
Jul 28, 2019·Current Atherosclerosis Reports·Sanjiv Singh, Avtar Singh Gautam
Jan 9, 2018·Cardiovascular Research·Celine SouilholGuido Krenning
May 20, 2016·PloS One·Chiharu YokoyamaShinya Minatoguchi
Nov 14, 2013·Journal of Cardiovascular Pharmacology·Yao DaiJawahar L Mehta
Mar 2, 2019·The Journal of Experimental Medicine·Torben MentrupBernd Schröder
Jul 25, 2019·Antioxidants·Ajoe John KattoorJawahar L Mehta
Sep 6, 2017·The Journal of Clinical Investigation·Ying WeiHarold A Chapman
Dec 31, 2020·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Izabela Tuleta, Nikolaos G Frangogiannis
Mar 4, 2021·European Journal of Pharmacology·Shiyuan XieBin Yang
Jul 3, 2021·Journal of Clinical Medicine·Sylwester RogulaKrzysztof J Filipiak
Aug 1, 2021·Advanced Drug Delivery Reviews·Izabela Tuleta, Nikolaos G Frangogiannis
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