Regulation of the aceI multidrug efflux pump gene in Acinetobacter baumannii

The Journal of Antimicrobial Chemotherapy
Qi LiuIan T Paulsen

Abstract

To investigate the function of AceR, a putative transcriptional regulator of the chlorhexidine efflux pump gene aceI in Acinetobacter baumannii. Chlorhexidine susceptibility and chlorhexidine induction of aceI gene expression were determined by MIC and quantitative real-time PCR, respectively, in A. baumannii WT and ΔaceR mutant strains. Recombinant AceR was prepared as both a full-length protein and as a truncated protein, AceR (86-299), i.e. AceRt, which has the DNA-binding domain deleted. The binding interaction of the purified AceR protein and its putative operator region was investigated by electrophoretic mobility shift assays and DNase I footprinting assays. The binding of AceRt with its putative ligand chlorhexidine was examined using surface plasmon resonance and tryptophan fluorescence quenching assays. MIC determination assays indicated that the ΔaceI and ΔaceR mutant strains both showed lower resistance to chlorhexidine than the parental strain. Chlorhexidine-induced expression of aceI was abolished in a ΔaceR background. Electrophoretic mobility shift assays and DNase I footprinting assays demonstrated chlorhexidine-stimulated binding of AceR with two sites upstream of the putative aceI promoter. Surface plasmon re...Continue Reading

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Citations

Aug 6, 2019·Frontiers in Microbiology·Faye C MorrisAnton Y Peleg
Jul 9, 2020·Proceedings of the National Academy of Sciences of the United States of America·Jani Reddy BollaCarol V Robinson
May 11, 2020·Current Opinion in Microbiology·Jessie L AllenLindsey N Shaw
Mar 25, 2021·Chemical Reviews·Peter J F HendersonKarl A Hassan
Apr 4, 2021·Pathogens·Meysam SarsharCecilia Ambrosi
Apr 28, 2021·Antimicrobial Agents and Chemotherapy·Vanessa Kornelsen, Ayush Kumar

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