Nov 1, 1978

Regulation of the activity of GTP-cyclohydrolase, the enzyme of the first step of flavinogenesis in yeasts

Biokhimii︠a︡
G M ShavlovskiĭV E Kashchenko

Abstract

The kinetic properties and regulation of activity of GTP-cyclohydrolase, the enzyme of the first step of flavinogenesis in the Pichia guilliermondii yests, partially purified by gel-filtration were studied. It was found that the curve of the dependence of reaction rate on substrate concentration is non-hyperbolic. FAD inhibited the enzyme activity, while riboflavin and FMN had no such effect. In addition to FAD, 5'-AMP, 3',5'-AMP, ADP, ATP, NAD and NADP inhibited the enzyme activity. Under combined action of FAD and AMP on GTP-cyclohydrolase no synergetic or antagonistic effects of the inhibitors on the enzyme activity were observed. The enzyme appreciably lost its sensitivity to FAD and AMP after thermal treatment. The data obtained suggest that GTP-cyclohydrolase from P. guilliermondii is an allosteric enzyme, which is inhibited by the end product of flavinogenesis FAD, as well as by other 5'-AMP-containing nucleotides.

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Mentioned in this Paper

Pichia guilliermondii
Filtration
Aminohydrolases
Yeasts
Riboflavin
Enzyme Activity
Blood Enzyme Activity (Lab Test)
Nucleotides
NADP
GTP Cyclohydrolase II

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