Regulation of the adenylyl cyclase signaling system in various types of cultured endothelial cells

Journal of Cellular Biochemistry
V G ManolopoulosP I Lelkes

Abstract

We studied the effects of modulators of the adenylyl cyclase pathway on the accumulation of cAMP in endothelial cells isolated from bovine aortas, pig pulmonary arteries, human umbilical veins, and human subcutaneous adipose microvessels. In addition to quantitative differences in the basal levels, cAMP stimulation in different endothelial cell types varied in sensitivity and magnitude in response to both the direct adenylyl cyclase activator forskolin and the beta-adrenergic receptor agonist isoproterenol. Furthermore, the ubiquitous phosphodiesterase inhibitor IBMX differentially enhanced both the basal and the stimulated cAMP levels in the various cell types. Histamine caused an elevation of cAMP only in bovine aortic endothelial cells and in human umbilical vein endothelial cells. Treatment of the cells with cholera and pertussis toxins, which uniquely affect G-protein subunits, resulted in divergent elevation of cAMP in the various cells. Thus, in each cell type, a distinct profile of regulation of the cAMP levels was found. Our results suggest that the adenylyl cyclase signaling system in various types of endothelial cells can be differentially regulated at the levels of receptors, G-proteins, adenylyl cyclase, and phosph...Continue Reading

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Citations

Jun 26, 2002·The Journal of Cell Biology·Donna L CioffiTroy Stevens
Jan 1, 1996·Journal of Biomaterials Science. Polymer Edition·V V NikolaychikP I Lelkes
Aug 20, 2005·Journal of Biomedical Materials Research. Part a·Pimpon UttayaratRussell J Composto
Feb 5, 2002·Endothelium : Journal of Endothelial Cell Research·E PapadimitriouP L Lelkes
Jul 3, 1999·Physiological Reviews·C C Michel, F E Curry
Feb 5, 2002·Endothelium : Journal of Endothelial Cell Research·E PapadimitriouP I Lelkes
Jul 1, 1997·The American Journal of Physiology·V G ManolopoulosB Nilius

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