Regulation of the Candida albicans cell wall damage response by transcription factor Sko1 and PAS kinase Psk1.

Molecular Biology of the Cell
Jason M RauceoAaron P Mitchell

Abstract

The environmental niche of each fungus places distinct functional demands on the cell wall. Hence cell wall regulatory pathways may be highly divergent. We have pursued this hypothesis through analysis of Candida albicans transcription factor mutants that are hypersensitive to caspofungin, an inhibitor of beta-1,3-glucan synthase. We report here that mutations in SKO1 cause this phenotype. C. albicans Sko1 undergoes Hog1-dependent phosphorylation after osmotic stress, like its Saccharomyces cerevisiae orthologues, thus arguing that this Hog1-Sko1 relationship is conserved. However, Sko1 has a distinct role in the response to cell wall inhibition because 1) sko1 mutants are much more sensitive to caspofungin than hog1 mutants; 2) Sko1 does not undergo detectable phosphorylation in response to caspofungin; 3) SKO1 transcript levels are induced by caspofungin in both wild-type and hog1 mutant strains; and 4) sko1 mutants are defective in expression of caspofungin-inducible genes that are not induced by osmotic stress. Upstream Sko1 regulators were identified from a panel of caspofungin-hypersensitive protein kinase-defective mutants. Our results show that protein kinase Psk1 is required for expression of SKO1 and of Sko1-dependent...Continue Reading

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Citations

Mar 2, 2010·Eukaryotic Cell·Jacob H BoysenAaron P Mitchell
Feb 9, 2010·PLoS Pathogens·Jill R BlankenshipAaron P Mitchell
Feb 24, 2012·PLoS Pathogens·Jonathan S FinkelAaron P Mitchell
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Apr 14, 2012·Current Biology : CB·Wenjie Xu, Aaron P Mitchell
Sep 23, 2009·Fungal Genetics and Biology : FG & B·Louise A WalkerCarol A Munro
Apr 15, 2010·Fungal Genetics and Biology : FG & B·Rebeca Alonso-MongeJesús Pla
Feb 27, 2015·Frontiers in Microbiology·Verónica UrrialdeRebeca Alonso-Monge
Nov 19, 2013·FEMS Microbiology Letters·Meixian HuangZhaojun Xu
Mar 8, 2019·MSphere·Jacky ChowPaul J Cullen
May 12, 2020·Frontiers in Genetics·Graham ThomasRyan M Ames
Nov 17, 2019·Journal of Fungi·Verónica UrrialdeRebeca Alonso-Monge
Apr 2, 2019·Frontiers in Microbiology·Julian C RutherfordChaoyang Xue
Aug 6, 2019·Frontiers in Cellular and Infection Microbiology·Alison M Day, Janet Quinn

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