Regulation of the cell surface expression and function of angiotensin II type 1 receptor by Rab1-mediated endoplasmic reticulum-to-Golgi transport in cardiac myocytes.

The Journal of Biological Chemistry
Catalin M FilipeanuGuangyu Wu

Abstract

Rab1 GTPase coordinates vesicle-mediated protein transport specifically from the endoplasmic reticulum (ER) to the Golgi apparatus. We recently demonstrated that Rab1 is involved in the export of angiotensin II (Ang II) type 1 receptor (AT1R) to the cell surface in HEK293 cells and that transgenic mice overexpressing Rab1 in the myocardium develop cardiac hypertrophy. To expand these studies, we determined in this report whether the modification of Rab1-mediated ER-to-Golgi transport can alter the cell surface expression and function of endogenous AT1R and AT1R-mediated hypertrophic growth in primary cultures of neonatal rat ventricular myocytes. Adenovirus-mediated gene transfer of wild-type Rab1 (Rab1WT) significantly increased cell surface expression of endogenous AT1R in neonatal cardiomyocytes, whereas the dominant-negative mutant Rab1N124I had the opposite effect. Brefeldin A treatment blocked the Rab1WT-induced increase in AT1R cell surface expression. Fluorescence analysis of the subcellular localization of AT1R revealed that Rab1 regulated AT1R transport specifically from the ER to the Golgi in HL-1 cardiomyocytes. Consistent with their effects on AT1R export, Rab1WT and Rab1N124I differentially modified the AT1R-media...Continue Reading

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Citations

Oct 30, 2012·The Journal of Biological Chemistry·Chunman LiGuangyu Wu
Feb 18, 2006·The Journal of Biological Chemistry·Catalin M FilipeanuGuangyu Wu
Apr 10, 2009·The Journal of Pharmacology and Experimental Therapeutics·Xiaoping ZhangGuangyu Wu
Jan 23, 2010·The Journal of Pharmacology and Experimental Therapeutics·Chunmin DongGuangyu Wu
Oct 22, 2011·Trends in Pharmacological Sciences·Guansong Wang, Guangyu Wu
Apr 8, 2006·Biochemical and Biophysical Research Communications·Yasuhito AbeKatsumi Kito
Dec 15, 2010·Biochimica Et Biophysica Acta·Catalin M FilipeanuDaniel R Kapusta
Nov 1, 2006·Biochimica Et Biophysica Acta·Chunmin DongGuangyu Wu
Jan 27, 2017·Small GTPases·Guansong WangGuangyu Wu
Jun 3, 2017·Journal of Cellular Physiology·Liqun LuLinxi Chen
Sep 9, 2006·The Journal of Biological Chemistry·Denis J DupréTerence E Hébert
Sep 6, 2011·American Journal of Physiology. Heart and Circulatory Physiology·George VaniotisTerence E Hébert
Oct 27, 2015·The Journal of Biological Chemistry·Surbhi BahlAmitabha Mukhopadhyay

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