Regulation of the CREB signaling cascade in the visual cortex by visual experience and neuronal activity

The Journal of Comparative Neurology
Seigo SuzukiTony A Pham

Abstract

The cAMP-responsive element (CRE) regulatory pathway has been studied as a model of signal-regulated transcription and is critical for some forms of learning and adaptation. In cell culture systems, the extracellular-regulated kinase (ERK) and ribosomal S6 kinase (RSK) couple synaptic signals to CRE-mediated gene expression by modulating CRE-binding protein (CREB) phosphorylation. However, it is not known whether sensory experience regulates gene expression in the brain by this mechanism. In this study, we ask: Are activated forms of ERK, RSK, and CREB colocalized in the cortex and are they coordinately regulated by synaptic signals? We find that these three signaling components are regulated in distinct ways. First, cells that show CRE-lacZ reporter expression, primarily excitatory neurons, do not colocalize with cells containing phospho-ERK. Second, while phosphorylation of ERK and RSK are modulated by visual experience, phosphorylation of CREB at serines 133, 142, or 143 is detected constitutively and is unaffected by experience. This finding suggests that neural activity might not regulate CREB phosphorylation in vivo. To test this hypothesis, we blocked action potentials by injection of tetrodotoxin and found no effect on ...Continue Reading

References

Mar 1, 1991·Visual Neuroscience·B R PayneS G Lomber
Nov 7, 1995·Proceedings of the National Academy of Sciences of the United States of America·P BrindleM Montminy
May 1, 1994·Journal of Neurophysiology·L M Kitzes, D Doherty
Oct 28, 1993·Nature·J C ChriviaR H Goodman
Oct 9, 1995·The Journal of Comparative Neurology·J W Lewis, J F Olavarria
Nov 21, 1996·Biochemical and Biophysical Research Communications·R Janknecht, A Nordheim
Dec 19, 1996·Nature·A J Bannister, T Kouzarides
Apr 16, 1998·Neuron·D H Hubel, T N Wiesel
Jun 11, 1999·The Journal of Biological Chemistry·K ObrietanD R Storm
Jun 26, 2001·Science·G Di CristoL Maffei
Aug 3, 2001·Nature Reviews. Molecular Cell Biology·B Mayr, M Montminy
Sep 6, 2001·Proceedings of the National Academy of Sciences of the United States of America·B M MayrM R Montminy
Sep 21, 2001·Molecular and Cellular Biology·K MerienneP Sassone-Corsi
Aug 28, 2002·The Journal of Biological Chemistry·Takahiro SuzukiHiroshi Ichinose
Oct 9, 2002·Neuron·Liliana MinichielloMartin Korte
Sep 25, 2003·Proceedings of the National Academy of Sciences of the United States of America·Vadim IourgenkoMark A Labow
Oct 11, 2003·Molecular Cell·Michael D ConkrightMarc Montminy

❮ Previous
Next ❯

Citations

Nov 4, 2008·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Daniela TropeaMriganka Sur
Nov 20, 2008·Genes, Brain, and Behavior·J LalondeA Chaudhuri
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Krisztián A KovácsJean-René Cardinaux
May 3, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Haiying DongXiaojie Zhang
Feb 19, 2008·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Camila Salum OliveiraFrancesco Mattia Rossi
Jun 15, 2007·The Journal of Comparative Neurology·Seigo SuzukiTony A Pham

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.