Regulation of the cytoplasmic accumulation of 5-methyltetrahydrofolate in MA104 cells is independent of folate receptor regulation.

The Journal of Clinical Investigation
B A KamenR G Anderson

Abstract

To better understand how the folate receptor (also known as the membrane folate binder) is able to deliver 5-methyltetrahydrofolic acid to the cytoplasm of folate-depleted MA104 cells, we have examined the kinetics of movement from the cell surface into the cytoplasm. Bound 5-methyltetrahydrofolic acid was transferred into an acid-resistant membrane compartment at the rate of 0.9-1.0 pmol/10(6) cells per h. This folate appeared in the cytoplasm at the same rate. Furthermore, cytoplasmic 5-methyltetrahydrofolic acid became polyglutamated at the rate of 0.6-0.7 pmol/10(6) cells per h. As soon as intracellular 5-methyltetrahydrofolate reached 5-7 pmol/10(6) cells, however, cytoplasmic accumulation was markedly inhibited even though the folate receptor remained functional. Therefore, the acute regulation of 5-methyltetrahydrofolic acid accumulation appears to be achieved by controlling the movement of the vitamin from the receptor into the cytoplasm of the cell.

References

Nov 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·B A Kamen, J D Caston
Nov 1, 1988·Proceedings of the Society for Experimental Biology and Medicine·E Sadasivan, S P Rothenberg
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·B A Kamen, A Capdevila
Jan 1, 1986·Annual Review of Biochemistry·G F Ames
Jan 1, 1974·Acta Haematologica·J D MantzosE Gyftaki
Jan 1, 1981·Annual Review of Biochemistry·C SennettI S Mellman
Jun 1, 1980·Archives of Biochemistry and Biophysics·G B HendersonF M Huennekens

❮ Previous
Next ❯

Citations

Aug 12, 2009·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Karensa K MenziesKevin R Nicholas
Mar 3, 2007·Cancer Metastasis Reviews·Marcela D'Alincourt Salazar, Manohar Ratnam
Jan 4, 1996·Biochimica Et Biophysica Acta·M da Costa, S P Rothenberg
Jul 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·C P Leamon, P S Low
Jul 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·H MatsueS W Lacey
Apr 25, 1995·Proceedings of the National Academy of Sciences of the United States of America·T E RitterS W Lacey
Jul 1, 1992·The Journal of Cell Biology·W J ChangR G Anderson
Jul 1, 1994·The Journal of Cell Biology·W J ChangR G Anderson
Nov 1, 1995·The Journal of Cell Biology·E J SmartR G Anderson
Sep 1, 1996·The Journal of Cell Biology·E J SmartR G Anderson
Aug 26, 1998·The EMBO Journal·S MayorF R Maxfield
Feb 1, 1994·Journal of Neurology, Neurosurgery, and Psychiatry·R A WeversP J Jongen
Oct 6, 1998·Annual Review of Biochemistry·R G Anderson
Apr 1, 1991·The Journal of Clinical Investigation·B A KamenR G Anderson
Dec 19, 2007·Journal of Neurochemistry·Jan B WollackBarton Kamen
Jul 21, 2011·International Journal of Pharmaceutics·Ahmed Al-KattanChristophe Drouet
Mar 6, 1998·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·R H FinnellJ A Piedrahita
Nov 10, 1995·The Journal of Biological Chemistry·P Liu, R G Anderson
May 17, 1996·The Journal of Biological Chemistry·C MineoR G Anderson
Jul 31, 2009·Biochemistry·Hala ElnakatManohar Ratnam
Feb 21, 1992·Cell·K G RothbergR G Anderson
Nov 1, 1992·The Journal of Investigative Dermatology·R G Anderson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.