PMID: 2522391Mar 15, 1989Paper

Regulation of the cytoplasmic pH by a proton-translocating ATPase in Streptococcus faecalis (faecium). A computer simulation

European Journal of Biochemistry
T Suzuki, H Kobayashi

Abstract

Earlier work from this laboratory led to the proposal that the cytoplasmic pH of streptococcal cells is regulated solely by changes in the amount and activity of a proton-translocating ATPase, F1F0 complex [Kobayashi, H., Suzuki, T. & Unemoto, T. (1986) J. Biol. Chem. 261, 627-630]. We have now examined the proposal with the aid of computer simulation. We find that an increase in the amount of the H+-ATPase is necessary for pH regulation and is sufficient to maintain a constant steady-state cytoplasmic pH. An increase in H+-ATPase activity is insufficient by itself to maintain a constant cytoplasmic pH, but suppresses the initial fluctuation of the pH. When both variations were allowed, the simulated cytoplasmic pH remained constant despite large perturbations, suggesting that this regulatory system has ample capacity to compensate for pH changes. The present work shows that a computer simulation is a useful way to examine a model for biological regulatory system; application of the simulation to other regulatory systems is discussed.

References

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Citations

Mar 1, 1994·Molecular Microbiology·M TakayamaH Kobayashi
Aug 12, 2000·Journal of Bacteriology·B J KoebmannP R Jensen
Dec 17, 2002·The Journal of General and Applied Microbiology·Hiroshi KobayashiTomohito Kakegawa
Jan 23, 1999·European Journal of Biochemistry·E ArikadoH Kobayashi
Dec 13, 2003·Biochimica Et Biophysica Acta·Hiroshi Kobayashi
Mar 3, 2010·Biochimica Et Biophysica Acta·David B HicksTerry A Krulwich
Oct 18, 2001·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·R G QuiveyK Hahn
Jun 1, 1997·European Journal of Biochemistry·P D Hockings, P J Rogers
Dec 16, 2011·Critical Reviews in Microbiology·Armand P Nyanga-KoumouDelphin Louembe
Sep 1, 1990·FEMS Microbiology Reviews·P C Maloney

Related Concepts

In Silico
Protoplasm
H(+)-Transporting ATP Synthase, Acyl-Phosphate-Linked
Hydrogen-Ion Concentration
Enterococcus faecalis

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