Regulation of the depth of surface liquid in bovine trachea

The American Journal of Physiology
D X WuJ H Widdicombe

Abstract

The luminal surface of airways is lined by a thin film of airway surface liquid (ASL). Physiological regulation of the depth of ASL has not been reported previously. In this paper, we have used low-temperature scanning electron microscopy of rapidly frozen specimens of bovine tracheal epithelium to demonstrate alterations in the depth of ASL in response to the cholinergic agonist methacholine. We first established that methacholine selectively stimulated airway glands, with maximal secretion at approximately 2 min and a return to baseline within approximately 5 min. A 2-min exposure to methacholine increased the depth of ASL from 23 to 78 microns. Thereafter, depth decreased linearly with time, reaching 32 microns at 30 min. The initial increase in depth was blocked by bumetanide, an inhibitor of active chloride secretion, whereas the slow decline back to baseline was inhibited by amiloride, a blocker of active sodium absorption. We conclude that the methacholine-induced changes in ASL depth reflect transient gland secretion followed by liquid absorption across the surface epithelium.

References

Dec 31, 1979·The Journal of Membrane Biology·J M Diamond
Sep 1, 1991·The American Review of Respiratory Disease·P Verdugo
Apr 1, 1990·Journal of Applied Physiology·Z V SeyboldA Wanner
Jan 1, 1990·Annual Review of Physiology·P Verdugo
Oct 1, 1988·Journal of Applied Physiology·C M YangT M Dwyer
Mar 1, 1988·The American Review of Respiratory Disease·M A SleighN Liron
Oct 1, 1987·Physiological Reviews·M J Welsh
Sep 1, 1984·Pflügers Archiv : European journal of physiology·J E Langridge-SmithM Field
Jun 1, 1982·Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology·B DavisJ A Nadel
Nov 1, 1980·Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology·M J WelshJ A Nadel
Dec 1, 1980·Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology·D B BorsonJ A Nadel
Aug 1, 1994·Pulmonary Pharmacology·H Rahmoune, K L Shephard
Jun 1, 1995·Journal of Applied Physiology·H Rahmoune, K L Shephard
Mar 1, 1994·Journal of Applied Physiology·K L Shephard, H Rahmoune
Jan 1, 1993·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·M KondoJ H Widdicombe
Jun 15, 1997·The Journal of Physiology·C JiangS S Miller

❮ Previous
Next ❯

Citations

Jun 3, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·E A CowleyD H Eidelman
Oct 16, 2001·American Journal of Physiology. Cell Physiology·S JayaramanA S Verkman
Dec 12, 2002·American Journal of Physiology. Cell Physiology·A S VerkmanJay R Thiagarajah
Mar 1, 2012·American Journal of Physiology. Lung Cellular and Molecular Physiology·J H WiddicombeSara Modlin
Dec 4, 2012·American Journal of Physiology. Lung Cellular and Molecular Physiology·Jeffrey L CooperStephen T Ballard
May 24, 2015·American Journal of Physiology. Lung Cellular and Molecular Physiology·Hyun-Chul ChoiRobert Tarran
Mar 9, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·R F DubinJ H Widdicombe
Sep 4, 2015·Physiological Reviews·Jonathan H Widdicombe, Jeffrey J Wine
Jul 4, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·N S JooJ J Wine
Jan 16, 2007·Annual Review of Medicine·Richard C Boucher
Apr 25, 2015·Biomedical Engineering Online·David E WhiteRoy J Nates
Apr 2, 2013·American Journal of Physiology. Lung Cellular and Molecular Physiology·Robert TarranSibylle Wilbert
Aug 17, 2002·Journal of Applied Physiology·Jonathan E PhillipsMichel R Corboz
Jan 11, 2003·British Journal of Pharmacology·Jonathan E PhillipsMichel R Corboz
Feb 4, 2021·The Journal of Experimental Medicine·Dusik KimJohn W Hanrahan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.