Regulation of the EGFR/ErbB signalling by clathrin in response to various ligands in hepatocellular carcinoma cell lines.

Journal of Cellular and Molecular Medicine
Yuanhui LiuFrançoise Praz

Abstract

Membrane receptor intracellular trafficking and signalling are frequently altered in cancers. Our aim was to investigate whether clathrin-dependent trafficking modulates signalling of the ErbB receptor family in response to amphiregulin (AR), EGF, heparin-binding EGF-like growth factor (HB-EGF) and heregulin-1β (HRG). Experiments were performed using three hepatocellular carcinoma (HCC) cell lines, Hep3B, HepG2 and PLC/PRF/5, expressing various levels of EGFR, ErbB2 and ErbB3. Inhibition of clathrin-mediated endocytosis (CME), by down-regulating clathrin heavy chain expression, resulted in a cell- and ligand-specific pattern of phosphorylation of the ErbB receptors and their downstream effectors. Clathrin down-regulation significantly decreased the ratio between phosphorylated EGFR (pEGFR) and total EGFR in all cell lines when stimulated with AR, EGF, HB-EGF or HRG, except in HRG-stimulated Hep3B cells in which pEGFR was not detectable. The ratio between phosphorylated ErbB2 and total ErbB2 was significantly decreased in clathrin down-regulated Hep3B cells stimulated with any of the ligands, and in HRG-stimulated PLC/PRF/5 cells. The ratio between phosphorylated ErbB3 and total ErbB3 significantly decreased in clathrin down-reg...Continue Reading

References

Mar 1, 1996·The Journal of Biological Chemistry·J BaulidaG Carpenter
Jun 5, 1998·The Journal of Biological Chemistry·H WatermanY Yarden
Jul 3, 2002·The EMBO Journal·Andrea H BildRichard Jove
Mar 22, 2003·Experimental Cell Research·Robert N JorissenAntony W Burgess
Oct 7, 2003·Molecular Cell·Antony W BurgessShigeyuki Yokoyama
Jan 27, 2004·Molecular Biology of the Cell·Anette M HommelgaardBo van Deurs
Feb 27, 2004·The Journal of Biological Chemistry·Fangtian HuangAlexander Sorkin
Oct 27, 2007·Annals of Oncology : Official Journal of the European Society for Medical Oncology·F RévillionJ-P Peyrat
Nov 21, 2007·Biochemical and Biophysical Research Communications·Fusanori YotsumotoShingo Miyamoto
Jun 24, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Masanori SeimiyaFumio Nomura
Mar 21, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Noemí Marina-GarcíaGabriel Núñez
Jun 18, 2009·Traffic·Kirstine RoepstorffBo van Deurs
Aug 14, 2009·Biochemistry·Aleksander Baldys, John R Raymond
Jul 7, 2010·Cell·Mark A Lemmon, Joseph Schlessinger
Apr 27, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Luca Di TommasoMassimo Roncalli
Jun 11, 2011·Biochimica Et Biophysica Acta·Benoit BusserAmandine Hurbin
Mar 23, 2012·Carcinogenesis·Malgorzata Magdalena SakInger Helene Madshus
Jul 24, 2012·The Journal of Biological Chemistry·Yu LiLinda J Pike
Jun 27, 2013·The EMBO Journal·Sara SigismundPier Paolo Di Fiore
Nov 26, 2013·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Robert Roskoski
Dec 4, 2013·Trends in Cell Biology·Alejandra TomasEmily R Eden
Dec 4, 2013·Cold Spring Harbor Perspectives in Biology·Ira Mellman, Yosef Yarden
Dec 10, 2013·Journal of Gastroenterology·Carmen Berasain, Matías A Avila
Jan 28, 2014·Seminars in Cell & Developmental Biology·Carmen Berasain, Matías A Avila
Apr 1, 2014·The International Journal of Biochemistry & Cell Biology·Sylwia Jones, Joshua Z Rappoport
Apr 3, 2014·Cold Spring Harbor Perspectives in Biology·Mark A LemmonKathryn M Ferguson
Aug 8, 2014·Membranes·Vibeke Bertelsen, Espen Stang
Sep 27, 2014·Growth Factors·Stine Louise Jeppe KnudsenLene Melsæther Grøvdal
Jun 10, 2015·Current Opinion in Cell Biology·Roshanak IrannejadMark von Zastrow

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Methods Mentioned

BETA
transfection
confocal microscopy
protein assay
imaging technology
nuclear translocation

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