Regulation of the epidermal growth factor receptor in fetal rat lung fibroblasts during late gestation

Endocrinology
C E L Dammann, H C Nielsen

Abstract

Lung epithelial cell differentiation is predominantly regulated by mesenchymal-epithelial cell communication. We have previously shown that epidermal growth factor (EGF) positively influences this process, and that EGF receptor (EGF-R) binding in fetal rat lung fibroblasts peaks on d18-19 of gestation, just before the onset of augmented surfactant synthesis. This regulation of EGF-R in late gestation fetal lung fibroblasts may control the timing of mesenchymal-epithelial cell communication leading to surfactant synthesis. Hormones and growth factors exert positive and negative influences on lung development, but whether they regulate the EGF-R is unknown. We hypothesized that positive [EGF, cortisol, retinoic acid (RA)] and negative [transforming growth-factor-beta1 (TGF-beta1), dihydrotestosterone (DHT)] regulators of lung cell development regulate the EGF-R in the fetal lung. We studied EGF-R binding and protein abundance in sex-specific fetal rat lung fibroblasts cultured at d17, d19, and d21. EGF-R binding was significantly elevated after RA (both sexes d17 and d19, females d21) and after DHT (females d19) treatment. EGF and cortisol had minimal or inhibitory effects on EGF-R binding. Western blot analysis showed that the o...Continue Reading

References

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Citations

Mar 7, 2006·Biochemical and Biophysical Research Communications·Susan M SmithMatt K Lee
Jan 28, 2006·American Journal of Respiratory and Critical Care Medicine·Barnaby WatersMike Silverman
Jun 17, 2008·American Journal of Respiratory and Critical Care Medicine·Brent ReyburnKurt H Albertine
Feb 5, 2009·Experimental Lung Research·Washa LiuChristiane E L Dammann
Mar 6, 2010·American Journal of Respiratory and Critical Care Medicine·Csaba GalambosHarry Kozakewich
Dec 31, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Sujatha M RamaduraiHeber C Nielsen
Feb 13, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Kwanchai ArchavachotikulMaryann V Volpe

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