Regulation of the epithelial Na+ channel by paraoxonase-2

The Journal of Biological Chemistry
Shujie ShiThomas R Kleyman


Paraoxonase-2 (PON-2) is a membrane-bound lactonase with unique anti-oxidative and anti-atherosclerotic properties. PON-2 shares key structural elements with MEC-6, an endoplasmic reticulum-resident molecular chaperone in Caenorhabditis elegans MEC-6 modulates the expression of a mechanotransductive ion channel comprising MEC-4 and MEC-10 in touch-receptor neurons. Because pon-2 mRNA resides in multiple rat nephron segments, including the aldosterone-sensitive distal nephron where the epithelial Na+ channel (ENaC) is expressed, we hypothesized that PON-2 would similarly regulate ENaC expression. We observed PON-2 expression in aquaporin 2-positive principal cells of the distal nephron of adult human kidney. PON-2 also co-immunoprecipitated with ENaC when co-expressed in HEK293 cells. When PON-2 was co-expressed with ENaC in Xenopus oocytes, ENaC activity was reduced, reflecting a reduction in ENaC surface expression. MEC-6 also reduced ENaC activity when co-expressed in Xenopus oocytes. The PON-2 inhibitory effect was ENaC-specific, as PON-2 had no effect on functional expression of the renal outer medullary potassium channel. PON-2 did not alter the response of ENaC to extracellular Na+, mechanical shear stress, or α-chymotryp...Continue Reading


Jun 25, 1996·Proceedings of the National Academy of Sciences of the United States of America·G GuMartin Chalfie
Apr 1, 1996·Current Opinion in Lipidology·M I MacknessR A Hegele
Dec 1, 1996·The American Journal of Physiology·H ZhouH Sackin
Nov 7, 1998·The Journal of Biological Chemistry·J A ValentijnC M Canessa
Dec 8, 1998·The Biochemical Journal·L S Prince, M J Welsh
Jan 26, 2002·The Journal of Biological Chemistry·He-Ping MaDavid G Warnock
May 29, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jeff H HogerAnne Hoger
Jun 28, 2002·Physiological Reviews·Stephan Kellenberger, Laurent Schild
Jul 3, 2003·The Journal of Biological Chemistry·Marcelo D CarattinoThomas R Kleyman
Mar 10, 2004·The Journal of Biological Chemistry·Rebecca P HugheyThomas R Kleyman
Apr 21, 2004·Nature Structural & Molecular Biology·Michal HarelDan S Tawfik
Sep 13, 2005·The Journal of Biological Chemistry·Oleh PochynyukJames D Stockand
Apr 6, 2006·Proceedings of the National Academy of Sciences of the United States of America·Samuel B GoldfarbRonald C Rubenstein
Apr 28, 2006·American Journal of Physiology. Renal Physiology·Tetsuji MorimotoThomas R Kleyman
May 13, 2006·The Journal of Biological Chemistry·Marcelo D CarattinoThomas R Kleyman
Apr 12, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Mike AlthausMartin Fronius
Aug 1, 2007·The Journal of Biological Chemistry·Ossama B KashlanThomas R Kleyman
Sep 20, 2007·The Journal of Biological Chemistry·Wanda H Vila-CarrilesDale J Benos
Sep 21, 2007·Nature·Jayasankar JastiEric Gouaux
Oct 6, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Emile LevyDaniel Sinnett
Mar 4, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Samira C GrifoniHeather A Drummond
Jul 25, 2008·The Journal of Biological Chemistry·Marcelo D CarattinoThomas R Kleyman
Aug 30, 2008·Journal of the American Society of Nephrology : JASN·Vivek Bhalla, Kenneth R Hallows
Jan 23, 2009·The Journal of Biological Chemistry·Ahmad B MaaroufShaohu Sheng
Feb 7, 2009·Nature Reviews. Molecular Cell Biology·Martin Chalfie
Apr 30, 2009·The Journal of Biological Chemistry·Thomas R KleymanRebecca P Hughey
Oct 21, 2009·The Journal of Biological Chemistry·David A StoltzJoseph Zabner
Jun 29, 2010·Antioxidants & Redox Signaling·Asokan DevarajanSrinivasa T Reddy
Nov 11, 2010·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Cesar Cortes-GonzálezNorma A Bobadilla
Jul 15, 2011·American Journal of Physiology. Renal Physiology·Ossama B Kashlan, Thomas R Kleyman
Oct 14, 2011·American Journal of Physiology. Renal Physiology·Christopher J PasseroThomas R Kleyman
Oct 20, 2011·Biochimica Et Biophysica Acta·Jill L Johnson
Mar 13, 2012·Experimental Cell Research·Ossama B Kashlan, Thomas R Kleyman
Apr 4, 2013·Current Molecular Pharmacology·Shujie ShiThomas R Kleyman
May 7, 2013·The Journal of Biological Chemistry·Teresa M BuckJeffrey L Brodsky
Jul 26, 2013·American Journal of Physiology. Cell Physiology·Rebecca A ChanouxRonald C Rubenstein
Jan 15, 2014·Nature Reviews. Nephrology·David G WarnockFrederic Jaisser
Jan 15, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Henning HagmannPaul Thomas Brinkkoetter
Jun 20, 2014·American Journal of Physiology. Cell Physiology·Yael GrumbachRonald C Rubenstein
Jul 21, 2014·Advances in Experimental Medicine and Biology·Asokan DevarajanSrinivasa T Reddy
Sep 12, 2014·American Journal of Physiology. Renal Physiology·Marcelo D CarattinoThomas R Kleyman
Mar 31, 2015·Journal of the American Society of Nephrology : JASN·Jae Wook LeeMark A Knepper
Sep 2, 2015·Proceedings of the National Academy of Sciences of the United States of America·Yushu ChenMartin Chalfie
Mar 26, 2016·Frontiers in Molecular Biosciences·Korrie L Mack, James Shorter
Feb 1, 2017·Journal of Cell Science·Hui YouXiubin Liang


Jun 20, 2018·Current Opinion in Nephrology and Hypertension·Teresa M Buck, Jeffrey L Brodsky
May 6, 2019·Antioxidants·Débora LevySérgio Paulo Bydlowski
Apr 26, 2018·American Journal of Physiology. Cell Physiology·Cristina MatthewmanLaura Bianchi
Feb 22, 2020·The Journal of Biological Chemistry·Shujie ShiThomas R Kleyman
Oct 28, 2019·American Journal of Physiology. Renal Physiology·Adam W WareFiona J McDonald

Related Concepts

PON1 protein, human
PON2 protein, human
MEC-6 protein, C elegans
Distal Convoluted Renal Tubule Structure
Conserved Sequence
Evolution, Molecular
Caenorhabditis elegans Proteins

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Related Papers

Pflügers Archiv : European journal of physiology
Dominik WiemuthStefan Gründer
Gravitational and Space Biology Bulletin : Publication of the American Society for Gravitational and Space Biology
M Driscoll, N Tavernarakis
© 2021 Meta ULC. All rights reserved