PMID: 2113912Jul 1, 1990Paper

Regulation of the Escherichia coli glyA gene by the purR gene product

Journal of Bacteriology
J G SteiertG V Stauffer

Abstract

The purine regulon repressor protein, PurR, was shown to be a purine component involved in glyA regulation in Escherichia coli. Expression of glyA, encoding serine hydroxymethyltransferase activity, was elevated in a purR mutant compared with a wild-type strain. When the purR mutant was transformed with a plasmid carrying the purR gene, the serine hydroxymethyltransferase levels returned to the wild-type level. The PurR protein bound specifically to a DNA fragment carrying the glyA control region, as determined by gel retardation. In a DNase I protection assay, a 24-base-pair region was protected from DNase I digestion by PurR. The glyA operator sequence for PurR binding is similar to that reported for several pur regulon genes.

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Citations

May 16, 1998·The Journal of Biological Chemistry·H XuK S Matthews
Jan 15, 1996·Molecular & General Genetics : MGG·E LorenzG V Stauffer
Nov 1, 1996·FEMS Microbiology Letters·E Lorenz, G V Stauffer
Jan 1, 1994·Antonie van Leeuwenhoek·J R Andreesen
Jul 28, 2019·Nature Communications·Zhi-Xue ChengXuan-Xian Peng
Apr 1, 1996·FEMS Microbiology Letters·E Lorenz, G V Stauffer
Jan 19, 2013·Database : the Journal of Biological Databases and Curation·Verena WeissJulio Collado-Vides
Jan 13, 2005·Canadian Journal of Microbiology·Neema T Chirwa, Muriel B Herrington
Jan 1, 1991·Progress in Biophysics and Molecular Biology·I G OldI Saint Girons
Dec 1, 2004·EcoSal Plus·George V Stauffer
Mar 1, 1994·Journal of Bacteriology·K Y Choi, H Zalkin
Jun 1, 1993·Journal of Bacteriology·P Nygaard, J M Smith
Jun 1, 1993·Journal of Bacteriology·B HeH Zalkin
Jul 1, 1995·Journal of Bacteriology·E Lorenz, G V Stauffer
Dec 1, 1993·Microbiological Reviews·M Riley

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