Regulation of the glucocorticoid receptor by glucocorticoids in human mononuclear leukocytes

Hormone Research
M M BrentaniV R Martins

Abstract

We analyzed glucocorticoid receptor binding in peripheral blood mononuclear leukocytes from normal adult males and from females at the follicular and luteal phases. Healthy controls were analyzed before and after 17 days of treatment with two synthetic glucocorticoids: prednisone and an oxazoline derivative of prednisolone (deflazacort). We also studied for comparison 4 patients with adrenocortical insufficiency, two of them on long-term corticoid replacement, and 7 patients with Cushing's syndrome. Using a whole-cell competitive binding assay and 3H-dexamethasone as tracer, normal human mononuclear leukocytes (19 males, 6 females) were found to have 4,529 +/- 1,532 (mean +/- SD) binding sites per cell and a dissociation constant (Kd) of 9.5 +/- 2.3 nM. In Cushing's syndrome the receptor parameters were within the normal range. Cells from patients with untreated Addison's disease had low levels of sites per cell. The number of binding sites increased to normal after long-term glucocorticoid replacement. All the adrenal insufficiency cases had a normal Kd. Finally, following treatment with the synthetic glucocorticoid, deflazacort, the sites per cell were reduced but the Kd remained unchanged. Prednisone had no effects.

Citations

Sep 29, 1989·Clinica Chimica Acta; International Journal of Clinical Chemistry·P Damm, C Binder
Oct 1, 1990·The Journal of Steroid Biochemistry and Molecular Biology·V R Martins, M M Brentani
Jun 1, 1992·The Journal of Steroid Biochemistry and Molecular Biology·M Alexandrová, P Farkas
Dec 17, 1998·Journal of Endocrinological Investigation·M GüvenG Kanigür-Sultuybek
Jan 1, 1990·Annals of the New York Academy of Sciences·M ScudelettiF Indiveri
Jun 23, 2004·International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity·A DovioA Angeli
Jan 1, 1993·Scandinavian Journal of Infectious Diseases·G A SigalM M Brentani

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Addison's disease, also known as primary adrenal insufficiency and hypocortisolism, is a long-term endocrine disorder in which the adrenal glands do not produce enough steroid hormones. Discover the latest research on Addison's disease here.