Regulation of the human endogenous retroviral Syncytin-1 and cell-cell fusion by the nuclear hormone receptors PPARγ/RXRα in placentogenesis

Journal of Cellular Biochemistry
Matthias RuebnerReiner Strick

Abstract

Cytotrophoblast (CT) cell fusion into a syncytiotrophoblast is obligatory for placentation and mediated by the human endogenous retrovirus (HERV)-W envelope gene Syncytin-1. Abnormal placentation is associated with preeclampsia (PE), HELLP and intrauterine growth restriction (IUGR). In placentogenesis, the MAP-kinase p38α regulates PPARγ/RXRα signaling and target genes, like leptin, resistin, ABCG2, and hCG. The aim of this study was to analyze PPARγ/RXRα signaling and target gene regulation using primary CT cultures, the trophoblastic cell line BeWo and placental tissues from patients with normal and abnormal placentation. CT from four different human control placentae and BeWo cells demonstrated that Syncytin-1, other signaling members and CT cell fusions were regulated with PPARγ/RXRα activators troglitazone and 9-cis retinoic acid, via protein kinase A and p38α inhibition. Significant discordant regulations between CTs and BeWo were found. Two PPARγ/RXRα-response-elements from upstream regulatory elements and the 5'LTR of HERV-W were confirmed with DNA-protein binding assays using nuclear extracts and recombinant PPARγ/RXRα proteins. These promoter elements were validated with luciferase assays in the presence of PPARγ/RXRα...Continue Reading

References

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Citations

Jun 29, 2013·Differentiation; Research in Biological Diversity·Christine HenkeReiner Strick
Sep 14, 2012·Reproductive Biology and Endocrinology : RB&E·Fabian B FahlbuschJörg Dötsch
Mar 12, 2013·PPAR Research·Satoru MatsudaYasuko Kitagishi
Aug 21, 2014·Cellular and Molecular Neurobiology·Alexander EmmerMalte E Kornhuber
Jul 9, 2014·Annual Review of Cell and Developmental Biology·Benjamin Podbilewicz
Jul 28, 2015·Animal Science Journal = Nihon Chikusan Gakkaihō·Katsuo KoshiKazuyoshi Hashizume
Apr 9, 2014·PPAR Research·Khrystyna LevytskaJohn Kingdom
Oct 16, 2016·Reproduction : the Official Journal of the Society for the Study of Fertility·Ru ZhengHongmei Wang
Nov 24, 2016·Journal of Biochemical and Molecular Toxicology·Yixin LinLauren M Aleksunes
Mar 19, 2014·Neuropathology and Applied Neurobiology·Rolf BusleiReiner Strick
Dec 20, 2017·Development·Javier M Hernández, Benjamin Podbilewicz
Dec 17, 2017·Reproductive Sciences·Hanna HuebnerMatthias Ruebner
Feb 23, 2020·Expert Opinion on Drug Metabolism & Toxicology·Ludwik Gorczyca, Lauren M Aleksunes
Aug 16, 2017·Cellular & Molecular Biology Letters·Sudha Saryu Malhotra, Satish Kumar Gupta
Jul 3, 2019·Reproductive Biology and Endocrinology : RB&E·Liang ZhaoLifang Sun
Jan 8, 2021·International Journal of Molecular Sciences·Lin PengViktoria von Schönfeldt
Feb 3, 2021·The FEBS Journal·Mingyu HuChao Tong
Jun 15, 2021·Human Reproduction Update·John D Aplin, Carolyn J P Jones

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