PMID: 9445010Jan 28, 1998Paper

Regulation of the human proliferating cell nuclear antigen promoter by the adenovirus E1A-associated protein p107

Journal of Virology
B H LeeM B Mathews

Abstract

The adenovirus E1A 243R oncoprotein is capable of transactivating the expression of the human proliferating cell nuclear antigen (PCNA) promoter. Mutational analysis of the E1A 243R protein suggested that both its p300/CBP- and p107-binding regions are required for optimal induction of the PCNA promoter (C. Kannabiran, G. F. Morris, C. Labrie, and M. B. Mathews, J. Virol. 67:425-437, 1993). We show that overexpression of p107 antagonizes the induction of PCNA by E1A 243R in transient expression assays. This inhibition is largely independent of p107's ability to interact with E1A 243R, because p107 mutants unable to bind to E1A 243R retain the ability to repress the E1A-activated PCNA promoter. Electrophoretic mobility shift assays with the PCNA promoter detected the presence of p107 in one of the major DNA-protein complexes, EH1, formed with HeLa cell nuclear extracts. Promoter mutations that disrupt the formation of complex EH1 abrogated p107's ability to reverse E1A 243R-induced PCNA expression. The same mutations characterize a sequence important for the binding of transcription factor RFX1 (C. Labrie, G. F. Morris, and M. B. Mathews, Nucleic Acids Res. 23:3732-3741, 1995), implying that p107 antagonizes E1A 243R-induced PCN...Continue Reading

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Citations

Dec 14, 1999·The Journal of Biological Chemistry·P K SenguptaB D Smith
May 3, 2002·The Journal of Biological Chemistry·Pritam K SenguptaBarbara D Smith
Sep 12, 1998·The Journal of Biological Chemistry·Y Katan-Khaykovich, Y Shaul
Apr 25, 2006·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Hirokazu SetoMasamitsu Yamaguchi
May 10, 2021·Cancer Cell International·Joby IssacAni V Das

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