We studied the properties of the permeability transition pore (PTP) in rat liver mitochondria and in mitoplasts retaining inner membrane ultrastructure and energy-linked functions. Like mitochondria, mitoplasts readily underwent a permeability transition following Ca(2+) uptake in a process that maintained sensitivity to cyclosporin A. On the other hand, major differences between mitochondria and mitoplasts emerged in PTP regulation by ligands of the outer membrane translocator protein of 18 kDa, TSPO, formerly known as the peripheral benzodiazepine receptor. Indeed, (i) in mitoplasts, the PTP could not be activated by photo-oxidation after treatment with dicarboxylic porphyrins endowed with protoporphyrin IX configuration, which bind TSPO in intact mitochondria; and (ii) mitoplasts became resistant to the PTP-inducing effects of N,N-dihexyl-2-(4-fluorophenyl)indole-3-acetamide and of other selective ligands of TSPO. Thus, the permeability transition is an inner membrane event that is regulated by the outer membrane through specific interactions with TSPO.
Isolation of the mitochondrial benzodiazepine receptor: association with the voltage-dependent anion channel and the adenine nucleotide carrier
Peripheral-type benzodiazepine receptors in endocrine organs: autoradiographic localization in rat pituitary, adrenal, and testis
Pyronin G as a fluorescent probe for quantitative determination of the membrane potential of mitochondria
Involvement of peripheral-type benzodiazepine receptors in the intracellular transport of heme and porphyrins
Mitochondrial benzodiazepine receptor linked to inner membrane ion channels by nanomolar actions of ligands
Modulation of the mitochondrial permeability transition pore by pyridine nucleotides and dithiol oxidation at two separate sites.
Complexes between kinases, mitochondrial porin and adenylate translocator in rat brain resemble the permeability transition pore
Oxidative stress, thiol reagents, and membrane potential modulate the mitochondrial permeability transition by affecting nucleotide binding to the adenine nucleotide translocase.
Singlet oxygen produced by photodynamic action causes inactivation of the mitochondrial permeability transition pore.
Characterization of binding sites for the omega3 receptor ligands [3H]PK11195 and [3H]RO5-4864 in human brain
The permeability transition pore complex: a target for apoptosis regulation by caspases and bcl-2-related proteins
PK11195, a ligand of the mitochondrial benzodiazepine receptor, facilitates the induction of apoptosis and reverses Bcl-2-mediated cytoprotection
Induction of the mitochondrial permeability transition by N-ethylmaleimide depends on secondary oxidation of critical thiol groups. Potentiation by copper-ortho-phenanthroline without dimerization of the adenine nucleotide translocase
Peripheral-type benzodiazepine receptor ligands: mitochondrial permeability transition induction in rat cardiac tissue
The effects of singlet oxygen produced by photodynamic action on the mitochondrial permeability transition differ in accordance with the localization of the sensitizer
Mitochondrial apoptosis and the peripheral benzodiazepine receptor: a novel target for viral and pharmacological manipulation
Effects on free radical generation by ligands of the peripheral benzodiazepine receptor in cultured neural cells
Translocator protein (18kDa): new nomenclature for the peripheral-type benzodiazepine receptor based on its structure and molecular function
The peripheral-type benzodiazepine receptor is involved in control of Ca2+-induced permeability transition pore opening in rat brain mitochondria
Peripheral benzodiazepine receptor ligand, PK11195 induces mitochondria cytochrome c release and dissipation of mitochondria potential via induction of mitochondria permeability transition
Hexokinase II detachment from mitochondria triggers apoptosis through the permeability transition pore independent of voltage-dependent anion channels.
Recent progress in elucidating the molecular mechanism of the mitochondrial permeability transition pore.
Bad targets the permeability transition pore independent of Bax or Bak to switch between Ca2+-dependent cell survival and death.
Switch from inhibition to activation of the mitochondrial permeability transition during hematoporphyrin-mediated photooxidative stress. Unmasking pore-regulating external thiols
Effect of peripheral benzodiazepine receptor (PBR/TSPO) ligands on opening of Ca2+-induced pore and phosphorylation of 3.5-kDa polypeptide in rat brain mitochondria
The mitochondria permeability transition pore complex in the brain with interacting proteins - promising targets for protection in neurodegenerative diseases
Mitofusins and the mitochondrial permeability transition: the potential downside of mitochondrial fusion.
Translocator protein (18 kDa): a promising therapeutic target and diagnostic tool for cardiovascular diseases
Rasagiline prevents apoptosis induced by PK11195, a ligand of the outer membrane translocator protein (18 kDa), in SH-SY5Y cells through suppression of cytochrome c release from mitochondria
Protective effect of Aloe vera gel on the permeability transition pore in the inner membrane of rat liver mitochondria in vitro
The mitochondrial permeability transition pore: molecular nature and role as a target in cardioprotection
Molecular identity of the mitochondrial permeability transition pore and its role in ischemia-reperfusion injury
The mitochondrial permeability transition: a current perspective on its identity and role in ischaemia/reperfusion injury
4'-Chlorodiazepam is neuroprotective against amyloid-beta through the modulation of survivin and bax protein expression in vitro
Enhanced oxidative stress sensitizes the mitochondrial permeability transition pore to opening in heart from Zucker Fa/fa rats with type 2 diabetes
Genetic analysis of dTSPO, an outer mitochondrial membrane protein, reveals its functions in apoptosis, longevity, and Ab42-induced neurodegeneration
Therapeutic actions of translocator protein (18kDa) ligands in experimental models of psychiatric disorders and neurodegenerative diseases
The development of mitochondrial membrane affinity chromatography columns for the study of mitochondrial transmembrane proteins
The translocator protein (peripheral benzodiazepine receptor) mediates rat-selective activation of the mitochondrial permeability transition by norbormide
Mixtures of glyphosate and surfactant TN20 accelerate cell death via mitochondrial damage-induced apoptosis and necrosis
The mitochondrial permeability transition pore is a dispensable element for mitochondrial calcium efflux
Interdependence of tetrapyrrole metabolism, the generation of oxidative stress and the mitigative oxidative stress response
Developmental expression of translocator protein/peripheral benzodiazepine receptor in reproductive tissues
Increased Susceptibility of Gracilinanus microtarsus Liver Mitochondria to Ca²⁺-Induced Permeability Transition Is Associated with a More Oxidized State of NAD(P)
Mitochondrial permeability transition pore (MPTP) desensitization increases sea urchin spermatozoa fertilization rate
Cardioprotective effects of intracoronary administration of 4-chlorodiazepam in small and large animal models of ischemia-reperfusion
Alterations in voltage-sensing of the mitochondrial permeability transition pore in ANT1-deficient cells
CRISPR/Cas9‒Mediated Tspo Gene Mutations Lead to Reduced Mitochondrial Membrane Potential and Steroid Formation in MA-10 Mouse Tumor Leydig Cells
Extent of mitochondrial hexokinase II dissociation during ischemia correlates with mitochondrial cytochrome c release, reactive oxygen species production, and infarct size on reperfusion
Poly (ADP-ribose) polymerase inhibition protects against myocardial ischaemia/reperfusion injury via suppressing mitophagy
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