Regulation of the insect immune response: the effect of hemolin on cellular immune mechanisms

Cellular Immunology
H Lanz-MendozaI Faye

Abstract

Hemolin is a bacteria-inducible protein of the immunoglobulin superfamily identified in the silk moth Hyalophora cecropia. The role of this protein, in hemocyte aggregation and phagocytosis, was studied in vitro. Hemocyte aggregation, stimulated by phorbol myristate acetate or lipopolysaccharide (LPS), was prevented by hemolin in a dose-dependent fashion, but hemolin did not disrupt aggregates once they had been formed. Furthermore, hemolin was able to stimulate phagocytic activity in both hemocytes and hemocytic mbn-2 cells and this activity was enhanced by LPS. The enhanced phagocytosis produced by a combination of hemolin and LPS was prevented by the protein kinase C (PKC) inhibitors staurosporine and H-7, and PKC activity in hemocyte crude extracts was enhanced by hemolin and LPS, with the highest activity observed in the presence of both. Hemolin affected tyrosine phosphorylation of hemocyte proteins, enhancing the phosphorylation of two proteins of 20 and 30 kDa and preventing tyrosine phosphorylation of two proteins of 35 and 40 kDa. These results suggest that hemolin is involved in the regulation of the cellular immune responses via a pathway that includes PKC activation and protein tyrosine phosphorylation.

Citations

Nov 13, 2008·Cytotechnology·Ronaldo Z MendonçaCarlos A Pereira
Dec 24, 2010·The Journal of Biological Chemistry·Miryam Paola Alvarez-FloresAna Marisa Chudzinski-Tavassi
Feb 14, 1998·European Journal of Biochemistry·R BettencourtI Faye
Mar 8, 2005·Immunology and Cell Biology·Cory L Brooks, Gary B Dunphy
Jan 1, 1997·Annual Review of Entomology·J P GillespieT Trenczek
Sep 12, 2009·Annual Review of Entomology·Otto SchmidtIngrid Faye
Apr 5, 2002·Journal of Medical Entomology·Salvador Hernández-MartínezVictor Tsutsumi
Nov 27, 2007·Developmental and Comparative Immunology·Lindsey S GarverGeorge Dimopoulos
May 21, 2009·Developmental and Comparative Immunology·Vladislav GuliiCraig A Mandato
Nov 30, 2005·Insect Molecular Biology·K Roxström-LindquistI Faye
Dec 29, 2005·Archives of Insect Biochemistry and Physiology·Dao-Jun ChengZhong-Huai Xiang
May 30, 2006·Journal of Insect Physiology·Małgorzata CytryńskaTeresa Jakubowicz
May 17, 2005·Journal of Insect Physiology·David MarinCraig A Mandato
Dec 21, 2014·Fish & Shellfish Immunology·Hongliang ZuoXiaopeng Xu
Aug 27, 2002·Fish & Shellfish Immunology·So Young Lee, Kenneth Söderhäll
Mar 31, 2015·International Immunopharmacology·Yu SunChao-Liang Liu
Dec 3, 2010·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Sony ShresthaYonggyun Kim
May 8, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·A J Nappi, E Ottaviani
Dec 3, 1999·European Journal of Biochemistry·R BettencourtI Faye
Oct 27, 1999·Archives of Insect Biochemistry and Physiology·X Q Yu, M R Kanost
Jul 14, 1998·Journal of Biomedical Science·J Y ChiouW L Cho
May 5, 2018·Scientific Reports·Veeranarayanan Surya AathmanathanMuthukalingan Krishnan
Nov 30, 2018·Pathogens·Lav Sharma, Guilhermina Marques
Jun 27, 2020·Insects·Maurizio Francesco Brivio, Maristella Mastore
Aug 14, 2020·Developmental and Comparative Immunology·Linwei YangXiaopeng Xu
Oct 29, 2000·Archives of Biochemistry and Biophysics·J R Meyer-FernandesM A Wells

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.