PMID: 2484577Jul 1, 1989Paper

Regulation of the isoforms of metallothionein

Biological Trace Element Research
C D Klaassen, L D Lehman-McKeeman

Abstract

The objective of this research was to study the regulation of hepatic MT-I and MT-II in rats. Induction of MTs following Cd, Zn, and glucocorticoid treatments was studied in adult rats and the ontogeny of MTs was evaluated in developing rats. The isoforms were quantitated by anion-exchange HPLC and their respective mRNAs were evaluated by Northern blot hybridization with mouse cRNA probes. In untreated adult rat liver, MT-II was more abundant than MT-I. Cd treatment increased MT-I and MT-II to similar concentrations. Low dosages of Zn increased MT-II levels three times higher than MT-I levels, whereas higher dosages increased the isoforms to similar levels. Differences in the degradation of Zn-induced MTs were observed: apparent half-lives for MT-I and MT-II were 12.2 and 21.9 h, respectively. MT-II was the predominant glucocorticoid-inducible isoform. MT-I and MT-II mRNAs increased coordinately after glucocorticoid treatment, but MT-II mRNA was more abundant than MT-I mRNA. In immature rats, MT-I and MT-II were first detected in fetal liver on gestation day 18 and increased to maximum hepatic concentrations during the first week postpartum. Thereafter, MT levels decreased, reaching adult levels by 35 d. In contrast, MT mRNAs r...Continue Reading

References

Sep 1, 1976·Toxicology and Applied Pharmacology·A P Leber, T S Miya
Jan 1, 1988·Toxicology and Applied Pharmacology·L D Lehman-McKeemanC D Klaassen
Jan 1, 1988·Toxicology and Applied Pharmacology·L D Lehman-McKeemanC D Klaassen
Jan 1, 1986·Annual Review of Biochemistry·D H Hamer
Apr 1, 1987·Toxicology and Applied Pharmacology·L D Lehman-McKeeman, C D Klaassen
Dec 11, 1974·Biochemical and Biophysical Research Communications·M G Cherian
Jan 1, 1983·Journal of Inherited Metabolic Disease·R J Cousins
Apr 5, 1983·European Journal of Biochemistry·J E PiletzH R Herschman
Aug 15, 1980·Biochemical Pharmacology·K T Suzuki, M Yamamura

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Citations

Jun 16, 2001·Toxicological Sciences : an Official Journal of the Society of Toxicology·E Canpolat, M A Lynes
Feb 9, 2002·The Journal of Comparative Neurology·Milena PenkowaJuan Hidalgo
Mar 20, 2019·International Journal of Molecular Sciences·Rhiannon V McNeillJennifer Southgate
Apr 8, 2000·Critical Reviews in Biochemistry and Molecular Biology·A T MilesV Rodilla
Jun 16, 2010·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Ivan SabolićCarol M Herak-Kramberger
Oct 27, 1999·Biological Trace Element Research·E BrambilaM Waalkes
Jul 25, 2000·Toxicology and Applied Pharmacology·K C CrowthersM A Lynes
Nov 25, 1998·Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology·S N PedersenM H Depledge
Feb 27, 2003·Biochemical and Biophysical Research Communications·Kayo YonezawaMinako Nagao

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