Regulation of the Marinomonas mediterranea antimicrobial protein lysine oxidase by L-lysine and the sensor histidine kinase PpoS.

Applied and Environmental Microbiology
Luisa R Molina-QuinteroAntonio Sánchez-Amat

Abstract

Some Gram-negative bacteria express a novel enzyme with lysine-epsilon-oxidase (LOD) activity (EC 1.4.3.20). The oxidation of l-Lys generates, among other products, hydrogen peroxide, which confers antimicrobial properties to this kind of enzyme and has been shown to be involved in cell death during biofilm development and differentiation. In addition to LOD, the melanogenic marine bacterium Marinomonas mediterranea, which forms part of the microbiota of the marine plant Posidonia oceanica, expresses two other oxidases of biotechnological interest, a multicopper oxidase, PpoA, with laccase activity and a tyrosinase named PpoB, which is responsible for melanin synthesis. By using both lacZ fusions with the lodAB promoter and quantitative reverse transcription-PCR (qRT-PCR), this study shows that the hybrid sensor histidine kinase PpoS regulates LOD activity at the transcriptional level. Although PpoS also regulates PpoA and PpoB, in this case, the regulatory effect cannot be attributed only to a transcriptional regulation. Further studies indicate that LOD activity is induced at the posttranscriptional level by l-Lys as well as by two structurally similar compounds, l-Arg and meso-2,6-diaminopimelic acid (DAP), neither of which ...Continue Reading

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Citations

Jun 8, 2012·Standards in Genomic Sciences·Patricia Lucas-ElíoAntonio Sanchez-Amat
Dec 30, 2015·Acta Biochimica Et Biophysica Sinica·Liuya ZhaoYuanying Jiang
Feb 24, 2016·Biofouling·Michela Gambino, Francesca Cappitelli
Jul 23, 2013·MicrobiologyOpen·Jonatan Cristian Campillo-BrocalAntonio Sanchez-Amat
Dec 14, 2018·Marine Drugs·Andrés Andreo-VidalJonatan C Campillo-Brocal
Apr 20, 2021·Biotechnology and Applied Biochemistry·Viviana Teresa OrlandiFabrizio Bolognese

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