Regulation of the metabolism of 25-hydroxyvitamin D3 in primary cultures of chick kidney cells

The Journal of Clinical Investigation
U TrechselH Fleisch

Abstract

A primary chick kidney cell culture is described, capable of forming 1,25-dihydroxyvitamin D3 [1,25(OH)2D3], 24,25-dihydroxyvitamin D3 [24,25(OH)2D3], and 1,24,25-trihydroxyvitamin D3 [1,24,25(OH)3D3] over several days. The apparent Km values were 0.125 microM for the 1-hydroxylase and 2.1 microM for the 24-hydroxylase. Exogenous 1,25(OH)2D3 decreased 1-hydroxylase and increased 24-hydroxylase within 4 h. 24,25(OH)2D3 produced similar effects, but only in the absence of fetal calf serum. R and S isomers of 1,24,25(OH)3D3 were about fives times less active than 1,25(OH)2D3. Bovine parathyroid hormone stimulated the 1- and reduced the 24-hydroxylase in 6 h, but this only occurred in cultures either previously treated with 1,25(OH)2D3 and EGTA to lower Ca to 0.8 mM or in cultures grown in the presence of 25-hydroxyvitamin D3 (25(OH)D3). Under the latter condition, the sensitivity to bovine parathyroid hormone was enhanced, 0.04 U/ml producing a maximum response. Synthetic aminoterminal tetratriacontapeptide (1-34) human parathyroid hormone was equally effective. In the absence of D metabolites, estradiol for 6 h produced a dose-dependent inhibition of the 1-hydroxylase, but no change in the 24-hydroxylase. Progesterone, testostero...Continue Reading

References

Sep 1, 1975·The Proceedings of the Nutrition Society·D R Fraser
Jan 1, 1976·Annual Review of Biochemistry·H F DeLuca, H K Schnoes
Mar 15, 1977·FEBS Letters·E SpanosI MacIntyre
Jan 24, 1977·Biochemical and Biophysical Research Communications·H L Henry
Feb 1, 1977·Archives of Biochemistry and Biophysics·L CastilloM L Sunde
Jun 21, 1976·Biochemical and Biophysical Research Communications·C M TaylorP de Silva
Sep 1, 1976·The Journal of Clinical Endocrinology and Metabolism·J G HaddadT Hahn
Aug 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·Y TanakaH F DeLuca
Mar 26, 1974·Biochemistry·J C Knutson, H F DeLuca
Sep 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·I T BoyleH F DeLuca
Jul 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·M GarabédianI T Boyle
Sep 1, 1972·The Journal of Clinical Investigation·H RasmussenD B Goodman
Jan 1, 1973·Calcified Tissue Research·J J ReynoldsH F De Luca
Nov 1, 1974·Analytical Biochemistry·A Leyva, W N Kelley
Feb 1, 1973·Archives of Biochemistry and Biophysics·Y Tanaka, H F DeLuca
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER
Jan 1, 1965·The Journal of Clinical Investigation·L G RAISZ

Citations

Aug 1, 1990·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·R W LettonH H Malluche
Sep 1, 1990·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J G Ghazarian
Oct 1, 1988·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·A Taylor, M E Norman
Jul 1, 1983·Calcified Tissue International·R T TurnerD J Baylink
Jan 1, 1981·Calcified Tissue International·G M Siu, H H Draper
May 1, 1991·Calcified Tissue International·C L SwensonL E Mathes
Sep 1, 1985·Calcified Tissue International·J E Puzas, J S Brand
Jul 1, 1988·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·C L BellC R Scriver
May 2, 1986·Biochimica Et Biophysica Acta·N S CunninghamH L Henry
May 1, 1994·Experimental Gerontology·L V Avioli
Jan 3, 1997·Molecular and Cellular Endocrinology·J Xu, H L Henry
Sep 23, 1998·The Journal of Steroid Biochemistry and Molecular Biology·A K NangiaR H Getzenberg
Feb 27, 2001·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·P L Dudas, J L Renfro
Aug 1, 1986·The Journal of Clinical Investigation·D D BikleP Elias
May 1, 1989·The Journal of Clinical Investigation·A A PortaleR C Morris
Oct 23, 1997·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·R St-ArnaudF H Glorieux
Apr 1, 1997·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·E G Seo, A W Norman
Sep 1, 1988·Kidney International·A DussoE Slatopolsky
Jan 1, 1986·Kidney International·H Kawashima, K Kurokawa
Jan 1, 1981·Metabolic Bone Disease & Related Research·H Fleisch
Jun 1, 1986·Metabolism: Clinical and Experimental·J R BuchananL M Demers
Mar 1, 1985·Journal of Oral Pathology·R D Finkelman, W T Butler
Dec 1, 1987·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·A B KorkorJ C Garancis
Apr 2, 2017·Journal of Molecular Endocrinology·Murat BastepeQing He
Jun 14, 2005·American Journal of Physiology. Renal Physiology·A DussoE Slatopolsky
Nov 2, 2006·American Journal of Physiology. Endocrinology and Metabolism·Yan ZhangMan-Sau Wong
Sep 3, 2016·Endocrine Reviews·Peter J TebbenRajiv Kumar
May 17, 2017·Journal of the American Veterinary Medical Association·Valerie J ParkerDennis J Chew
Jan 1, 1984·The American Journal of Physiology·H J ArmbrechtB B Davis
Aug 1, 1980·The American Journal of Physiology·U TrechselH Fleisch
Aug 13, 1999·The American Journal of Physiology·A J BrownE Slatopolsky

Related Concepts

Calcium
High Pressure Liquid Chromatography Procedure
Dihydroxycholecalciferols
Hormones
Hydroxycholecalciferols
Kidney
Parathyroid Hormone Peptide (1-34)
Red Phosphorus
Didronel
Steroid Hydroxylases

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