Regulation of the miR-19b-mediated SOCS6-JAK2/STAT3 pathway by lncRNA MEG3 is involved in high glucose-induced apoptosis in hRMECs.

Bioscience Reports
Fan XiaoRong Luo

Abstract

Diabetic retinopathy (DR) is one of the most severe and common complications of diabetes mellitus. The present study aimed to investigate the molecular mechanism of MEG3, miR-19b and SOCS6 in human retinal microvascular endothelial cells (hRMECs) under high glucose conditions. HRMECs were cultured in 5 or 30 mM D-glucose medium. qRT-PCR and Western blotting were used to determine the mRNA expression and protein levels. MTT assay and flow cytometry analysis were performed to detect the viability and apoptosis of hRMECs, respectively. TNF-α, IL-6 and IL-1β levels in cell supernatants were detected by ELISA. The activity of caspase-3/7 was also determined. A luciferase reporter assay was performed to confirm the targeting relationship between miR-19b and SOCS6, as well as MEG3 and miR-19b. Our study demonstrated that miR-19b was increased and SOCS6 was decreased in HG-induced hRMECs. Knockdown of SOCS6 inhibited cell viability and reversed the promotion of cell viability induced by knockdown of miR-19b. Additionally, miR-19b directly targeted and negatively regulated SOCS6. Moreover, miR-19b promoted the cell apoptosis rate and caspase-3/7 activity and increased inflammatory factors through the SOCS6-mediated JAK2/STAT3 signalling...Continue Reading

References

Jan 28, 2014·Acta Pharmacologica Sinica·El-shaimaa A ArafaHany A Omar
Feb 5, 2016·Biochemical and Biophysical Research Communications·Gui-Zhen QiuBan Liu
Oct 19, 2017·Scientific Reports·Camila Uribe CopierSebastián D Calligaris
Jan 10, 2018·Frontiers in Microbiology·Skyla A DuncanVida A Dennis
Jun 22, 2018·International Journal of Molecular Sciences·Wei Wang, Amy C Y Lo
Nov 21, 2018·World Journal of Surgical Oncology·Soudeh Ghafouri-FardMohammad Taheri
Jan 8, 2019·Experimental Eye Research·Johanna M WalzAndreas Stahl
Apr 19, 2019·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Li YinDelong Zhang
May 12, 2019·Bioscience Reports·Lian-Jiao Quan, Wen-Jun Wang
May 18, 2019·The British Journal of Ophthalmology·Eva K FenwickEcosse Luc Lamoureux
Jun 12, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Xiaoliang LiZhigang Yang
Jun 20, 2019·Cellular and Molecular Life Sciences : CMLS·Delin KongChun-Bo Teng
Jul 23, 2019·Journal of Cellular and Molecular Medicine·Xiaowen ZhangZhijie Li
Aug 15, 2019·Journal of Cellular and Molecular Medicine·Guoqing LiDan Liu
Sep 24, 2019·Investigative Ophthalmology & Visual Science·Ebony LiuKathryn P Burdon

❮ Previous
Next ❯

Citations

Feb 6, 2021·Clinica Chimica Acta; International Journal of Clinical Chemistry·Shun ZhangWujun Chen
Apr 4, 2021·International Journal of Molecular Sciences·Hsiuying Wang

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
Protein Assay
transfection
Flow cytometry
ELISA

Software Mentioned

SPSS
ImageJ

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis